E-selectin /CD62E Polyclonal antibody
E-selectin /CD62E Polyclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 20894-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, L02 cells, HUVEC cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HUVEC cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 12 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 20894-1-AP is E-selectin /CD62E Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | E-selectin /CD62E fusion protein Ag14944 |
Full Name | selectin E |
Calculated molecular weight | 610 aa, 67 kDa |
Observed molecular weight | 67 kDa |
GenBank accession number | BC142711 |
Gene symbol | SELE |
Gene ID (NCBI) | 6401 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)
Protocols
Product Specific Protocols | |
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WB protocol for E-selectin /CD62E antibody 20894-1-AP | Download protocol |
IHC protocol for E-selectin /CD62E antibody 20894-1-AP | Download protocol |
FC protocol for E-selectin /CD62E antibody 20894-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Antioxid Redox Signal GENDER DIFFERENCE IN DAMAGE MEDIATED SIGNALING CONTRIBUTES TO PULMONARY ARTERIAL HYPERTENSION. | ||
Cell Death Dis MicroRNA-181a-5p and microRNA-181a-3p cooperatively restrict vascular inflammation and atherosclerosis. | ||
Graefes Arch Clin Exp Ophthalmol CD40 ligand induces expression of vascular cell adhesion molecule 1 and E-selectin in orbital fibroblasts from patients with Graves' orbitopathy. | ||
Acta Biochim Biophys Sin (Shanghai) SET8 is involved in the regulation of hyperglycemic memory in human umbilical endothelial cells. | ||
J Cardiovasc Pharmacol Ther Chrysin Suppresses Vascular Endothelial Inflammation via Inhibiting the NF-κB Signaling Pathway. | ||
Biosci Biotechnol Biochem A hot water extract of Curcuma longa inhibits adhesion molecule protein expression and monocyte adhesion to TNF-α-stimulated human endothelial cells. |