E-selectin /CD62E Polyclonal antibody

E-selectin /CD62E Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 20894-1-AP

Synonyms

CD62E, ELAM, ELAM 1, ELAM1, ESEL, E-selectin, LECAM2, SELE, selectin E



Tested Applications

Positive WB detected inJurkat cells, L02 cells, HUVEC cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20894-1-AP is E-selectin /CD62E Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-selectin /CD62E fusion protein Ag14944
Full Name selectin E
Calculated molecular weight 610 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC142711
Gene symbol SELE
Gene ID (NCBI) 6401
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

Protocols

Product Specific Protocols
WB protocol for E-selectin /CD62E antibody 20894-1-APDownload protocol
IHC protocol for E-selectin /CD62E antibody 20894-1-APDownload protocol
FC protocol for E-selectin /CD62E antibody 20894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Antioxid Redox Signal

GENDER DIFFERENCE IN DAMAGE MEDIATED SIGNALING CONTRIBUTES TO PULMONARY ARTERIAL HYPERTENSION.

Authors - Ruslan Rafikov
humanWB

Cell Death Dis

MicroRNA-181a-5p and microRNA-181a-3p cooperatively restrict vascular inflammation and atherosclerosis.

Authors - Yingxue Su
human

Graefes Arch Clin Exp Ophthalmol

CD40 ligand induces expression of vascular cell adhesion molecule 1 and E-selectin in orbital fibroblasts from patients with Graves' orbitopathy.

Authors - Hao Wang
humanWB

Acta Biochim Biophys Sin (Shanghai)

SET8 is involved in the regulation of hyperglycemic memory in human umbilical endothelial cells.

Authors - Xiangyuan Chen
humanWB

J Cardiovasc Pharmacol Ther

Chrysin Suppresses Vascular Endothelial Inflammation via Inhibiting the NF-κB Signaling Pathway.

Authors - Shengnan Zhao
WB

Biosci Biotechnol Biochem

A hot water extract of Curcuma longa inhibits adhesion molecule protein expression and monocyte adhesion to TNF-α-stimulated human endothelial cells.

Authors - Kengo Kawasaki