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E-selectin /CD62E Antibody 11 Publications

Rabbit Polyclonal| Catalog number: 20894-1-AP

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Con: 22 μg/150 μl

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Tested Applications

Applications:
WB, IHC, FC, ELISA
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Images See all

E-selectin /CD62E Antibody WB Jurkat cells 20894-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 20894-1-AP(E-selectin /CD62E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

E-selectin /CD62E Antibody IHC human tonsillitis tissue 20894-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

E-selectin /CD62E Antibody IHC human tonsillitis tissue 20894-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

E-selectin /CD62E Antibody FC HUVEC cells 20894-1-AP

1X10^6 HUVEC cells were stained with 0.2ug E-selectin /CD62E antibody (20894-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, L02 cells, HUVEC cells
Positive IHC detected in:
human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
HUVEC cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:200-1:800
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
E-selectin /CD62E fusion protein Ag14944
Full name:
selectin E
Calculated molecular weight:
610aa,67 kDa
Observed molecular weight:
67 kDa
GenBank accession number:
BC142711
Gene ID (NCBI):
6401
Gene symbol
SELE
Synonyms
CD62E, ELAM, ELAM 1, ELAM1, ESEL, E-selectin, LECAM2, SELE, selectin E

Background

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)


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