E-selectin /CD62E Polyclonal antibody
E-selectin /CD62E Polyclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 20894-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, L02 cells, HUVEC cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 12 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 20894-1-AP is E-selectin /CD62E Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E-selectin /CD62E fusion protein Ag14944 |
| Full Name | selectin E |
| Calculated molecular weight | 610 aa, 67 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC142711 |
| Gene symbol | SELE |
| Gene ID (NCBI) | 6401 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for E-selectin /CD62E antibody 20894-1-AP | Download protocol |
| IHC protocol for E-selectin /CD62E antibody 20894-1-AP | Download protocol |
| FC protocol for E-selectin /CD62E antibody 20894-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Antioxid Redox Signal GENDER DIFFERENCE IN DAMAGE MEDIATED SIGNALING CONTRIBUTES TO PULMONARY ARTERIAL HYPERTENSION. | ||
Cell Death Dis MicroRNA-181a-5p and microRNA-181a-3p cooperatively restrict vascular inflammation and atherosclerosis. | ||
Graefes Arch Clin Exp Ophthalmol CD40 ligand induces expression of vascular cell adhesion molecule 1 and E-selectin in orbital fibroblasts from patients with Graves' orbitopathy. | ||
Acta Biochim Biophys Sin (Shanghai) SET8 is involved in the regulation of hyperglycemic memory in human umbilical endothelial cells. | ||
J Cardiovasc Pharmacol Ther Chrysin Suppresses Vascular Endothelial Inflammation via Inhibiting the NF-κB Signaling Pathway. | ||
Biosci Biotechnol Biochem A hot water extract of Curcuma longa inhibits adhesion molecule protein expression and monocyte adhesion to TNF-α-stimulated human endothelial cells. |
