HUVEC cells were subjected to SDS PAGE followed by western blot with 20894-1-AP( E-selectin /CD62E antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 20894-1-AP(E-selectin /CD62E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

L02 cells were subjected to SDS PAGE followed by western blot with 20894-1-AP(E-selectin /CD62E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

1X10^6 HUVEC cells were stained with 0.2ug E-selectin /CD62E antibody (20894-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.