|Positive WB detected in||human peripheral blood platelets, Jurkat cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
60322-1-Ig targets P-selectin in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||P-selectin fusion protein Ag4124|
|Full Name||selectin P (granule membrane protein 140kDa, antigen CD62)|
|Calculated molecular weight||830 aa, 91 kDa|
|Observed molecular weight||140 kDa, 105-110 kDa|
|GenBank accession number||BC028067|
|Gene ID (NCBI)||6403|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
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