Tested Applications
| Positive WB detected in | human peripheral blood platelets, Jurkat cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60322-1-Ig targets P-selectin/CD62P in WB, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4124 Product name: Recombinant human SELP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 441-789 aa of BC028067 Sequence: VCQALQCQDLPVPNEARVNCSHPFGAFRYQSVCSFTCNEGLLLVGASVLQCLATGNWNSVPPECQAIPCTPLLSPQNGTMTCVQPLGSSSYKSTCQFICDEGYSLSGPERLDCTRSGRWTDSPPMCEAIKCPELFAPEQGSLDCSDTRGEFNVGSTCHFSCNNGFKLEGPNNVECTTSGRWSATPPTCKGIASLPTPGVQCPALTTPGQGTMYCRHHPGTFGFNTTCYFGCNAGFTLIGDSTLSCRPSGQWTAVTPACRAVKCSELHVNKPIAMNCSNLWGNFSYGSICSFHCLEGQLLNGSAQTACQENGHWSTTVPTCQDDGKCPLNPHSHLGTYGVFTNAAFDPSP Predict reactive species |
| Full Name | selectin P (granule membrane protein 140kDa, antigen CD62) |
| Calculated Molecular Weight | 830 aa, 91 kDa |
| Observed Molecular Weight | 140 kDa, 105-110 kDa |
| GenBank Accession Number | BC028067 |
| Gene Symbol | CD62P |
| Gene ID (NCBI) | 6403 |
| RRID | AB_2881433 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16109 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for P-selectin/CD62P antibody 60322-1-Ig | Download protocol |
| WB protocol for P-selectin/CD62P antibody 60322-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The P-selectin/CD62P Monoclonal antibody (3G2C7), catalog number 60322-1-Ig, has 48 total citations. These appear in journals including Cell Metabolism, Science Advances, Advanced Science, ACS Applied Materials & Interfaces, Acta Biomaterialia, Journal of Controlled Release, International Journal of Biological Macromolecules, Biomaterials Advances, and iScience, among others. Research fields span platelet biology and thrombosis, biomimetic nanomedicine for cancer therapy, cardiovascular disease (stroke, myocardial infarction), inflammation (ulcerative colitis, asthma), and tissue repair.
| Species | Application | Title |
|---|---|---|
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Exploration (Beijing) Photodynamic Biomimetic Nanoparticles Accelerate Tumor Vascular Normalization Initiation. | ||
Sci Bull (Beijing) Engineered platelets-based nano-aircraft system for precise tumor chemo-immunotherapy with graded drug delivery and self-recognized tumor targeting | ||
Adv Sci (Weinh) Remodeling Collagen Microenvironment in Liver Using a Biomimetic Nano-Regulator for Reversal of Liver Fibrosis | ||
Sci Adv Platelet-derived vesicles restore fertility in endometrial injury by modulating the endometrial immune niche in mice. | ||
J Control Release Platelet-mimetic nano-sensor for combating postoperative recurrence and wound infection of triple-negative breast cancer |
