P-selectin Monoclonal antibody

P-selectin Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G2C7

Cat no : 60322-1-Ig

Synonyms

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP



Tested Applications

Positive WB detected inhuman peripheral blood platelets, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60322-1-Ig targets P-selectin in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P-selectin fusion protein Ag4124
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Calculated molecular weight 830 aa, 91 kDa
Observed molecular weight 140 kDa, 105-110 kDa
GenBank accession numberBC028067
Gene symbol SELP
Gene ID (NCBI) 6403
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Protocols

Product Specific Protocols
WB protocol for P-selectin antibody 60322-1-IgDownload protocol
IHC protocol for P-selectin antibody 60322-1-IgDownload protocol
IP protocol for P-selectin antibody 60322-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Int J Biol Macromol

Low molecular weight fucoidan modulates P-selectin and alleviates diabetic nephropathy.

Authors - Yingjie Xu
mouseWB

Front Bioeng Biotechnol

Nano-Platelets as an Oxygen Regulator for Augmenting Starvation Therapy Against Hypoxic Tumor.

Authors - Chunyu Huang