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SERPINA6/CBG Polyclonal antibody

SERPINA6/CBG Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12010-1-AP

Synonyms

CBG, Serpin A6, SERPINA6, Transcortin

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    WB analysis of human plasma using 12010-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    WB analysis of L02 using 12010-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    WB analysis of A375 using 12010-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    WB analysis of HEK-293 using 12010-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human kidney using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human kidney using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human liver using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human liver using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human hepatocirrhosis using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human hepatocirrhosis using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human liver cancer using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human liver cancer using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human liver cancer using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IHC staining of human liver cancer using 12010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPINA6/CBG Polyclonal antibody (12010-1-AP)

    IF Staining of HepG2 using 12010-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12010-1-AP (SERPINA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma tissue, A375 cells, HEK-293 cells, L02 cells
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue, human liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12010-1-AP targets SERPINA6/CBG in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPINA6/CBG fusion protein Ag2623
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
Calculated Molecular Weight 405 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC058021
Gene Symbol SERPINA6
Gene ID (NCBI) 866
RRIDAB_874108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA6, also known as corticosteroid-binding globulin or CBG, belongs to the serpin family of serine protease inhibitors, and is mainly secreted by the liver. It is an alpha-globulin protein with corticosteroid-binding properties, and involved in obesity and stress sensitivity. It is also the major transport protein for glucorticoids and progestins in the blood of most vertebrates. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency).

Protocols

Product Specific Protocols
WB protocol for SERPINA6/CBG antibody 12010-1-APDownload protocol
IHC protocol for SERPINA6/CBG antibody 12010-1-APDownload protocol
IF protocol for SERPINA6/CBG antibody 12010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm.

Authors - Acosta-Martin Adelina E AE
View Article