SERPINA6 Polyclonal antibody

SERPINA6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12010-1-AP

Synonyms

CBG, Serpin A6, SERPINA6, Transcortin



Tested Applications

Positive WB detected inhuman plasma tissue, A375 cells, HEK-293 cells, L02 cells
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue, human liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12010-1-AP targets SERPINA6 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPINA6 fusion protein Ag2623
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
Calculated molecular weight 405 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC058021
Gene symbol SERPINA6
Gene ID (NCBI) 866
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINA6, also known as corticosteroid-binding globulin or CBG, belongs to the serpin family of serine protease inhibitors, and is mainly secreted by the liver. It is an alpha-globulin protein with corticosteroid-binding properties, and involved in obesity and stress sensitivity. It is also the major transport protein for glucorticoids and progestins in the blood of most vertebrates. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency).

Protocols

Product Specific Protocols
WB protocol for SERPINA6 antibody 12010-1-APDownload protocol
IHC protocol for SERPINA6 antibody 12010-1-APDownload protocol
IF protocol for SERPINA6 antibody 12010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm.

Authors - Acosta-Martin Adelina E AE