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  • KD/KO Validated

SH3PXD2A Polyclonal antibody

SH3PXD2A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18976-1-AP

Synonyms

Adapter protein TKS5, FISH, KIAA0418, SH3 and PX domains 2A, SH3 multiple domains protein 1, SH3MD1, SH3PXD2A, TKS5



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

18976-1-AP targets SH3PXD2A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SH3 and PX domains 2A
Calculated molecular weight 125 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberNM_014631
Gene symbol SH3PXD2A
Gene ID (NCBI) 9644
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH3PXD2A is also named as FISH, KIAA0418, SH3MD1, TKS5. SH3PXD2A contains an amino-terminal PX domain followed by five SH3 domains. It is a cytoplasmic protein in normal fibroblasts (PMID: 19464300). The p140 and p130 forms of SH3PXD2A may be generated by other splice variations. The complexity of SH3PXD2A isoforms is also evident in different cell types. For example in human platelets a single band of 150 kDa was detected, whereas all three forms were found in human fibroblasts and vascular smooth muscle cells (PMID: 9687503). This antibody is specific to SH3PXD2A.

Protocols

Product Specific Protocols
WB protocol for SH3PXD2A antibody 18976-1-APDownload protocol
IHC protocol for SH3PXD2A antibody 18976-1-APDownload protocol
IF protocol for SH3PXD2A antibody 18976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Death Dis

Association of lncRNA SH3PXD2A-AS1 with preeclampsia and its function in invasion and migration of placental trophoblast cells.

Authors - Qian Chen
humanWB, IHC

PLoS One

The invadopodia scaffold protein tks5 is required for the growth of human breast cancer cells in vitro and in vivo.

Authors - Barbara Blouw
humanIHC

Prostate

Src-dependent Tks5 phosphorylation regulates invadopodia-associated invasion in prostate cancer cells.

Authors - Burger Karen L KL
humanWB

Oncotarget

The role of Tks adaptor proteins in invadopodia formation, growth and metastasis of melanoma.

Authors - Shinji Iizuka
  • KD Validated

Theranostics

IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis.

Authors - Ge Liu
mouseIF

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma