Filter: AllWBIHCIF/ICC

WB analysis using 12641-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12641-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 12641-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12641-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12641-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 12641-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of rat brain using 12641-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12641-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 12641-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 12641-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of HepG2 using 12641-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

SHC4 Polyclonal antibody

Name: SHC4 Polyclonal antibody
Brand: Proteintech
SKU: 12641-1-AP

SHC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12641-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

SHC transforming protein 4, SH2 domain protein C4, RaLP, Rai-like protein, Rai like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, human brain tissue, HepG2 cells, human skeletal muscle tissue, mouse brain tissue, A375 cells, rat brain tissue
Positive IHC detected in	rat brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 2 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

12641-1-AP targets SHC4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3336 Product name: Recombinant human SHC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 324-630 aa of BC033907 Sequence: NGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEVHIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKCSSVYENCLEQSRAIGNVHPRGVQSQRGTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGNQRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLLVKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDNSLPIISSGSEVSLKQPVRKDNNPALLHSNK Predict reactive species
Full Name	SHC (Src homology 2 domain containing) family, member 4
Calculated Molecular Weight	630 aa, 69 kDa
Observed Molecular Weight	69 kDa
GenBank Accession Number	BC033907
Gene Symbol	SHC4
Gene ID (NCBI)	399694
RRID	AB_10643243
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6S5L8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for SHC4 antibody 12641-1-AP	Download protocol
IHC protocol for SHC4 antibody 12641-1-AP	Download protocol
WB protocol for SHC4 antibody 12641-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Clin Sci (Lond) Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations. Authors - Zhiling Luo View Article
human	IHC,IF	PeerJ A new 12-gene diagnostic biomarker signature of melanoma revealed by integrated microarray analysis. Authors - Liu Wanting W View Article
mouse,human	WB,IHC,IF,CoIP	Cancer Lett SHC4 orchestrates β-catenin pathway-mediated metastasis in triple-negative breast cancer by promoting Src kinase autophosphorylation Authors - Wenjing Zhong KD Validated View Article