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  • KD/KO Validated

SLC30A8/ZNT8-Specific Polyclonal antibody

SLC30A8/ZNT8-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16169-1-AP

Synonyms

SLC30A8, Zinc transporter 8, ZnT 8, ZNT8



Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman pancreas tissue, human pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16169-1-AP targets SLC30A8/ZNT8-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name solute carrier family 30 (zinc transporter), member 8
Calculated molecular weight 41 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_173851
Gene symbol SLC30A8
Gene ID (NCBI) 169026
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC30A8 (also named as ZNT8) encods the secretory granule zinc transporter, permiting cellular efflux of zinc. SLC30A8 was recently defined as a autoantigen candidates of type 2 diabetes, although the molecular mechanism through which this phenotype is manifest is incompletely understood.

Protocols

Product Specific Protocols
WB protocol for SLC30A8/ZNT8-Specific antibody 16169-1-APDownload protocol
IHC protocol for SLC30A8/ZNT8-Specific antibody 16169-1-APDownload protocol
IF protocol for SLC30A8/ZNT8-Specific antibody 16169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Invest Ophthalmol Vis Sci

Erythropoietin Protects Retinal Cells in Diabetic Rats Through Upregulating ZnT8 via Activating ERK Pathway and Inhibiting HIF-1α Expression.

Authors - Guoxu Xu
  • KD Validated
humanIHC

Sci Rep

A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells.

Authors - Shogo Ohta
mouseWB

J Biol Chem

A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells.

Authors - Chengfeng Merriman

J Biol Chem

Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8.

Authors - Chengfeng Merriman
humanWB

J Biol Chem

Lipid-tuned Zinc Transport Activity of Human ZnT8 Correlates with Risk for Type-2 Diabetes.

Authors - Chengfeng Merriman
ratWB

J Biol Chem

PMID: 28130446

Authors - Qiong Huang