SMAD1 Polyclonal antibody

SMAD1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10429-1-AP

Synonyms

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, SMAD 1, SMAD family member 1, SMAD1



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 8 publications below

Product Information

10429-1-AP targets SMAD1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD1 fusion protein Ag0701
Full Name SMAD family member 1
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC001878
Gene symbol SMAD1
Gene ID (NCBI) 4086
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase

Protocols

Product Specific Protocols
WB protocol for SMAD1 antibody 10429-1-APDownload protocol
IF protocol for SMAD1 antibody 10429-1-APDownload protocol
IP protocol for SMAD1 antibody 10429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway.

Authors - Qi Cui
mouseWB

BMC Oral Health

ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum.

Authors - Jingyu Wang
mouseWB

BMC Pulm Med

IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation.

Authors - Zhaoxing Dong
mouseWB

Neuroscience

LRG1 Promotes Apoptosis and Autophagy through the TGFβ-smad1/5 Signaling Pathway to Exacerbate Ischemia/Reperfusion Injury.

Authors - Jing Jin
mouseWB

Sci Rep

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.

Authors - Xueni Wang
mouseWB

Ecotoxicol Environ Saf

Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.

Authors - Di Wang