SMAD1 Monoclonal antibody

SMAD1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D10B8

Cat no : 66559-1-Ig

Synonyms

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, SMAD 1, SMAD family member 1, SMAD1



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells
Positive IHC detected inhuman gliomas tissue,
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66559-1-Ig targets SMAD1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMAD1 fusion protein Ag0701
Full Name SMAD family member 1
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC001878
Gene symbol SMAD1
Gene ID (NCBI) 4086
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase

Protocols

Product Specific Protocols
WB protocol for SMAD1 antibody 66559-1-IgDownload protocol
IHC protocol for SMAD1 antibody 66559-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.

Authors - Jinliang Chen