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SMAD2 Polyclonal antibody

SMAD2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12570-1-AP

Synonyms

hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2



Tested Applications

Positive WB detected inHepG2 cells, multi-cells, Jurkat cells, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman endometrial cancer tissue, human heart tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12570-1-AP targets SMAD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity C. tubulosa, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD2 fusion protein Ag3237
Full Name SMAD family member 2
Calculated molecular weight 467 aa, 52 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC014840
Gene symbol SMAD2
Gene ID (NCBI) 4087
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAD2. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)

Protocols

Product Specific Protocols
WB protocol for SMAD2 antibody 12570-1-APDownload protocol
IHC protocol for SMAD2 antibody 12570-1-APDownload protocol
IF protocol for SMAD2 antibody 12570-1-APDownload protocol
IP protocol for SMAD2 antibody 12570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Authors - Li Yan
humanWB

EBioMedicine

POH1 contributes to hyperactivation of TGF-β signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-β receptors and caveolin-1.

Authors - Boshi Wang
ratWB

J Ethnopharmacol

Raw and vinegar processed Curcuma wenyujin regulates hepatic fibrosis via bloking TGF-β/Smad signaling pathways and up-regulation of MMP-2/TIMP-1 ratio.

Authors - Hui Xie
IF

Cancer Res

A feedback loop between hypoxia and matrix stress relaxation increases oxygen-axis migration and metastasis in sarcoma.

Authors - Daniel M Lewis
mouseWB

Mol Med Rep

Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction.

Authors - Jin Zhao
humanWB

Gynecol Oncol

Platelets promote invasion and induce epithelial to mesenchymal transition in ovarian cancer cells by TGF-β signaling pathway.

Authors - Yi Guo