SMAD4 Polyclonal antibody

SMAD4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10231-1-AP

Synonyms

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4



Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, HepG2 cells, MCF-7 cells, mouse liver tissue, NIH/3T3 cells, SMMC-7721 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman placenta tissue, human testis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10231-1-AP targets SMAD4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity Bovine, goat, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD4 fusion protein Ag0299
Full Name SMAD family member 4
Calculated molecular weight 60 kDa
Observed molecular weight 63-70 kDa
GenBank accession numberBC002379
Gene symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.

Protocols

Product Specific Protocols
WB protocol for SMAD4 antibody 10231-1-APDownload protocol
IHC protocol for SMAD4 antibody 10231-1-APDownload protocol
IP protocol for SMAD4 antibody 10231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,WB

Am J Physiol Endocrinol Metab

Myostatin / miR-124-3p / GR signaling pathway regulates intramuscular fat deposition in mice.

Authors - Kaiqing Liu
ratWB

Arch Gynecol Obstet

Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling.

Authors - Xiaohua Li
humanWB

Brain Res Bull

miR-93-5p targets Smad7 to regulate the transforming growth factor-β1/Smad3 pathway and mediate fibrosis in drug-resistant prolactinoma.

Authors - Bin Hu
humanWB

J Cell Mol Med

The role and mechanism of transforming growth factor beta 3 in human myocardial infarction-induced myocardial fibrosis.

Authors - Ke Xue
humanWB,IHC

Brain Res

Role of TGF-β1/Smad3-mediated fibrosis in drug resistance mechanism of prolactinoma.

Authors - Bin Hu
BovineWB

J Exp Zool B Mol Dev Evol

Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.

Authors - Xin-Xin Zhao