|Positive WB detected in||HEK-293 cells, A549 cells, HepG2 cells, MCF-7 cells, mouse liver tissue, NIH/3T3 cells, SMMC-7721 cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human placenta tissue, human testis tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10231-1-AP targets SMAD4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||Bovine, goat, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SMAD4 fusion protein Ag0299|
|Full Name||SMAD family member 4|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||63-70 kDa|
|GenBank accession number||BC002379|
|Gene ID (NCBI)||4089|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.
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