Tested Applications
| Positive WB detected in | A431 cells, mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14185-1-AP targets SMC3 in WB, IHC, IF/ICC, CoIP, RIP, ELISA, CUT&Tag applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5440 Product name: Recombinant human SMC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC047324 Sequence: MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNLFYAIQFVLSDEFSHLRPEQRLALLHEGTGPRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVKQGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEELAQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTKISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEP Predict reactive species |
| Full Name | structural maintenance of chromosomes 3 |
| Calculated Molecular Weight | 142 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC047324 |
| Gene Symbol | SMC3 |
| Gene ID (NCBI) | 9126 |
| RRID | AB_2192625 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQE7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SMC3 antibody 14185-1-AP | Download protocol |
| IHC protocol for SMC3 antibody 14185-1-AP | Download protocol |
| WB protocol for SMC3 antibody 14185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SMC3 Polyclonal antibody (Cat# 14185-1-AP) has 9 citations published in journals including Nature Communications, PNAS, Cell Reports, Cell Mol Life Sci, Int J Mol Sci, Oncol Res, Biology (Basel), Heliyon, and Curr Eye Res. Research fields span epigenetics and acute myeloid leukemia, germ cell development, pancreatic cancer, stem cell biology, protein interactomics, breast cancer, RNA stress granules, and ophthalmology.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeting SMC3 deacetylation synergizes with XPO1 inhibition to reprogram the epigenetic landscape and suppress NPM1-mutated acute myeloid leukemia | ||
Proc Natl Acad Sci U S A Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in Trachemys scripta | ||
Cell Rep Regulatory elements in the Sox9 locus license the initiation of pancreatic ductal adenocarcinoma. | ||
Cell Mol Life Sci RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells | ||
Oncol Res Targeting Aurora A Kinase Enhance the CDK4/6 Inhibitor Sensitivity in HR+/HER2- Breast Cancer. |
