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SMC3 Polyclonal antibody

SMC3 Polyclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14185-1-AP

Synonyms

BAM, Bamacan, BMH, CDLS3, CSPG6, HCAP, SMC 3, SMC protein 3, SMC3, SMC3L1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SMC3 Polyclonal antibody (14185-1-AP)

    WB analysis of HeLa using 14185-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SMC3 Polyclonal antibody (14185-1-AP)

    WB analysis of Jurkat using 14185-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SMC3 Polyclonal antibody (14185-1-AP)

    WB analysis of mouse brain using 14185-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SMC3 Polyclonal antibody (14185-1-AP)

    WB analysis of Jurkat using 14185-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of Jurkat cells using SMC3 Polyclonal antibody (14185-1-PBS)

    WB analysis of Jurkat using 14185-1-PBS

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMC3 Polyclonal antibody (14185-1-AP)

    IF Staining of HeLa using 14185-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14185-1-AP (SMC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using SMC3 Polyclonal antibody (14185-1-AP)

    FC experiment of HeLa using 14185-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SMC3 (14185-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse brain tissue
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14185-1-AP targets SMC3 in WB, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMC3 fusion protein Ag5440
Full Name structural maintenance of chromosomes 3
Calculated Molecular Weight 142 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC047324
Gene Symbol SMC3
Gene ID (NCBI) 9126
RRIDAB_2192625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SMC3 antibody 14185-1-APDownload protocol
IF protocol for SMC3 antibody 14185-1-APDownload protocol
FC protocol for SMC3 antibody 14185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Int J Mol Sci

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

Authors - Marianna Naki
View Article
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
View Article