CL488-conjugated SMN-Exon7 Antibody

SMN-Exon7 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3A8G11

Cat no : CL488-60255

Synonyms

Component of gems 1, Gemin 1, SMA, SMA@, SMA1, SMA2, SMA3, SMA4, SMN, SMN1, SMN2, SMNC, SMNT, Survival motor neuron protein, T BCD541



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60255-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The CL488-60255 immunogen is Fusion Protein CL488-conjugated SMN-Exon7 protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMN-Exon7 fusion protein Ag16615
Full Name survival of motor neuron 1, telomeric
Calculated molecular weight 294aa,32 kDa
Observed molecular weight 40 kDa
GenBank accession number BC062723
Gene symbol SMN1
Gene ID (NCBI) 6606
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated SMN-Exon7 antibody CL488-60255Download protocol
Standard Protocols
Click here to view our Standard Protocols