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  • Featured Product
  • KD/KO Validated

SMOX Polyclonal antibody

SMOX Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15052-1-AP

Synonyms

C20orf16, PAO, PAO 1, PAOh1, Polyamine oxidase 1, SMO, SMOX, spermine oxidase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMOX Polyclonal antibody (15052-1-AP)

    WB analysis using 15052-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-13 cells using SMOX Polyclonal antibody (15052-1-AP)

    WB analysis of PC-13 using 15052-1-AP

    PC-13 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SMOX Polyclonal antibody (15052-1-AP)

    WB analysis of MCF-7 using 15052-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human prostate cancer using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human prostate cancer using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human stomach cancer using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human stomach cancer using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human gliomas using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human gliomas using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, PC-13 cells, MCF-7 cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human stomach cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15052-1-AP targets SMOX in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMOX fusion protein Ag7035
Full Name spermine oxidase
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 56-65 kDa
GenBank Accession NumberBC000669
Gene Symbol SMOX
Gene ID (NCBI) 54498
RRIDAB_2239689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMOX(Spermine oxidase) is also named as SMO, PAO-1 and PAOh1. It belongs to the class of polyamine oxidases (PAOs).The flavoprotein SMO, which specifically oxidizes Spm, is one of the key regulatory enzymes involved in polyamine homeostasis in animal cells (PMID:14764092). It has 6 isoforms produced by alternative splicing with the MW of 61 kDa, 56 kDa, 21 kDa, 60 kDa, 43 kDa and 65 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for SMOX antibody 15052-1-APDownload protocol
IHC protocol for SMOX antibody 15052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma.

Authors - Anna Bianchi-Smiraglia
View Article
mouseWB

J Clin Invest

Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.

Authors - Leslie A Sandusky-Beltran
View Article
mouseWB

EBioMedicine

Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.

Authors - Guangtian Wang
View Article
humanWB

J Invest Dermatol

The Polyamine Putrescine Promotes Human Epidermal Melanogenesis.

Authors - Aishwarya Sridharan
View Article

Free Radic Biol Med

Adaptive responses of heart and skeletal muscle to spermine oxidase overexpression: Evaluation of a new transgenic mouse model.

Authors - Roberta Ceci
View Article
humanWB,IHC

Biomedicines

Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients.

Authors - Sooyoun Kim
View Article