Tested Applications
| Positive WB detected in | A549 cells, PC-13 cells, MCF-7 cells, HeLa cells, PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue, human gliomas tissue, human stomach cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15052-1-AP targets SMOX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7035 Product name: Recombinant human SMOX protein Source: e coli.-derived, PKG Tag: GST Domain: 1-280 aa of BC000669 Sequence: MQSCESSGDSADDPLSRGLRRRGQPRVVVIGAGLAGLAAAKALLEQGFTDVTVLEASSHIGGRVQSVKLGHATFELGATWIHGSHGNPIYHLAEANGLLEETTDGERSVGRISLYSKNGVACYLTNHGRRIPKDVVEEFSDLYNEVYNLTQEFFRHDKPVNAESQNSVGVFTREEVRNRIRNDPDDPEATKRLKLAMIQQYLKVESCESSSHSMDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPAHVIQLGKPVRCIHWDQASARPRGPEIEP Predict reactive species |
| Full Name | spermine oxidase |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 56-65 kDa |
| GenBank Accession Number | BC000669 |
| Gene Symbol | SMOX |
| Gene ID (NCBI) | 54498 |
| RRID | AB_2239689 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NWM0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMOX(Spermine oxidase) is also named as SMO, PAO-1 and PAOh1. It belongs to the class of polyamine oxidases (PAOs).The flavoprotein SMO, which specifically oxidizes Spm, is one of the key regulatory enzymes involved in polyamine homeostasis in animal cells (PMID:14764092). It has 6 isoforms produced by alternative splicing with the MW of 61 kDa, 56 kDa, 21 kDa, 60 kDa, 43 kDa and 65 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMOX antibody 15052-1-AP | Download protocol |
| WB protocol for SMOX antibody 15052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SMOX (Spermine Oxidase) antibody (Cat# 15052-1-AP) has accumulated 47 citations across journals including Nature, Nat Commun, J Clin Invest, Cancer Research, Cancer Letters, EBioMedicine, Scientific Reports, International Journal of Molecular Sciences, Biomedicines, and PLoS ONE. Associated research fields encompass polyamine metabolism, ferroptosis, oncology (colorectal, lung, breast, pancreatic cancers), neurodegeneration, ischemic stroke, retinal diseases, kidney fibrosis, skeletal muscle physiology, inflammation, rheumatoid arthritis, diabetes, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Cancer Res Spermine Oxidase Serves as a Key Functional Node in Microbial Dysbiosis-Induced Breast Carcinogenesis. | ||
Nat Commun P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance | ||
J Clin Invest Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma. | ||
