SNAI2 Polyclonal antibody

SNAI2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12129-1-AP

Synonyms

Protein snail homolog 2, SLUG, SLUGH, SLUGH1, SNAI2, snail homolog 2 (Drosophila), WS2D, Zinc finger protein SNAI2



Tested Applications

Positive WB detected inmouse brain tissue, mouse spleen tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

12129-1-AP targets SNAI2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNAI2 fusion protein Ag2773
Full Name snail homolog 2 (Drosophila)
Calculated molecular weight 268 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC015895
Gene symbol SNAI2
Gene ID (NCBI) 6591
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

Protocols

Product Specific Protocols
WB protocol for SNAI2 antibody 12129-1-APDownload protocol
IHC protocol for SNAI2 antibody 12129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p.

Authors - Wei Wu
humanWB

Cancer Manag Res

Lipase member H is a downstream molecular target of hypoxia inducible factor-1α and promotes papillary thyroid carcinoma cell migration in BCPAP and KTC-1 cell lines.

Authors - Yuefeng Li
humanWB

Theranostics

Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme.

Authors - Yaodong Chen
humanWB

J Exp Clin Cancer Res

Nuciferine inhibits the progression of glioblastoma by suppressing the SOX2-AKT/STAT3-Slug signaling pathway.

Authors - Zizhuo Li
humanWB,IF

Cell Death Dis

M2 macrophages promote NSCLC metastasis by upregulating CRYAB.

Authors - Zhe Guo
humanWB

Onco Targets Ther

Nr5a2 promotes tumor growth and metastasis of gastric cancer AGS cells by Wnt/beta-catenin signaling.

Authors - Lei Liu