SNAP23 Antibody

SNAP23 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10825-1-AP

Synonyms

HsT17016, SNAP 23, SNAP23, SNAP23A, SNAP23B



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10825-1-AP targets SNAP23 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity goat, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNAP23 fusion protein Ag1269
Full Name synaptosomal-associated protein, 23kDa
Calculated molecular weight 23 kDa
Observed molecular weight 23-25 kDa
GenBank accession number BC000148
Gene symbol SNAP23
Gene ID (NCBI) 8773
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SNAP23 antibody 10825-1-APDownload protocol
IHC protocol for SNAP23 antibody 10825-1-APDownload protocol
IF protocol for SNAP23 antibody 10825-1-APDownload protocol
IP protocol for SNAP23 antibody 10825-1-APDownload protocol
FC protocol for SNAP23 antibody 10825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Cancer

RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer.

Authors - Wei-Chung Cheng
goatIHC

Eur J Histochem

Ultrastructure and immunohistochemical characterization of proteins concerned with the secretory machinery in goat ceruminous glands.

Authors - Tadashi Yasui
ratWB,IHC

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
humanWB

J Lipid Res

Syntaxin 17 promotes lipid droplet formation by regulating the distribution of acyl-CoA synthetase 3.

Authors - Hana Kimura
ratWB

Front Cell Infect Microbiol

Comparison of the Expression Changes after Botulinum Toxin Type A and Minocycline Administration in Lipopolysaccharide-Stimulated Rat Microglial and Astroglial Cultures.

Authors - Anna Piotrowska
humanWB,IF

Nat Commun

KRAS regulation by small non-coding RNAs and SNARE proteins.

Authors - Yonglu Che