SNRPB2 Polyclonal antibody

SNRPB2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13512-1-AP

Synonyms

SNRPB2, U2 snRNP B



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse lung tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13512-1-AP targets SNRPB2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRPB2 fusion protein Ag4436
Full Name small nuclear ribonucleoprotein polypeptide B''
Calculated molecular weight 225 aa, 26, 5 kDa
Observed molecular weight 26.5 kDa
GenBank accession numberBC036737
Gene symbol SNRPB2
Gene ID (NCBI) 6629
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

Protocols

Product Specific Protocols
WB protocol for SNRPB2 antibody 13512-1-APDownload protocol
IHC protocol for SNRPB2 antibody 13512-1-APDownload protocol
IF protocol for SNRPB2 antibody 13512-1-APDownload protocol
IP protocol for SNRPB2 antibody 13512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Sci Transl Med

Comment on "Drug screening for ALS using patient-specific induced pluripotent stem cells".

Authors - Bilada Bilican
humanIF

Sci Transl Med

Drug screening for ALS using patient-specific induced pluripotent stem cells.

Authors - Naohiro Egawa
humanWB

Cell Chem Biol

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Authors - Li Wang
humanWB

Nucleic Acids Res

Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways.

Authors - Li Chen
humanWB

Nature

CPEB1 coordinates alternative 3'-UTR formation with translational regulation.

Authors - Felice-Alessio Bava

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Melissa (Verified Customer) (03-16-2020)

This antibody works incredibly robust in western blot analysis. However, we do observe a non-specific band in all of our blots which we have attributed to snRPA due to the size and sequence similarity of the immunogen.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa Cell Nuclear Extract
FH

John (Verified Customer) (12-06-2018)

  • Applications: Western Blot,
  • Cell Tissue Type: HeLa
SNRPB2 Antibody Western Blot, validation ( dilution) in HeLa (Cat no:13512-1-AP)