|Positive WB detected in||HeLa cells, human brain tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse lung tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13512-1-AP targets SNRPB2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SNRPB2 fusion protein Ag4436|
|Full Name||small nuclear ribonucleoprotein polypeptide B''|
|Calculated molecular weight||225 aa, 26, 5 kDa|
|Observed molecular weight||26.5 kDa|
|GenBank accession number||BC036737|
|Gene ID (NCBI)||6629|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.
|Product Specific Protocols|
|WB protocol for SNRPB2 antibody 13512-1-AP||Download protocol|
|IHC protocol for SNRPB2 antibody 13512-1-AP||Download protocol|
|IF protocol for SNRPB2 antibody 13512-1-AP||Download protocol|
|IP protocol for SNRPB2 antibody 13512-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Melissa (Verified Customer) (03-16-2020)
This antibody works incredibly robust in western blot analysis. However, we do observe a non-specific band in all of our blots which we have attributed to snRPA due to the size and sequence similarity of the immunogen.
John (Verified Customer) (12-06-2018)