Tested Applications
| Positive WB detected in | HEK-293 cells, HEK-293T cells, HeLa cells, SH-SY5Y cells, Neuro-2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, Jurkat cells, human placenta tissue |
| Positive IP detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HEK-293 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10269-1-AP targets SOD1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, chicken, bovine, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0335 Product name: Recombinant human SOD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC001034 Sequence: MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ Predict reactive species |
| Full Name | superoxide dismutase 1, soluble |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC001034 |
| Gene Symbol | SOD1 |
| Gene ID (NCBI) | 6647 |
| RRID | AB_2193750 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00441 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IHC protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IP protocol for SOD1 antibody 10269-1-AP | Download protocol |
| WB protocol for SOD1 antibody 10269-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SOD1 polyclonal antibody (Cat# 10269-1-AP) has accumulated 441 citations published in journals such as Nature Communications, Cell Metabolism, Redox Biology, Free Radical Biology and Medicine, Antioxidants, Oxidative Medicine and Cellular Longevity, Advanced Science, Biomaterials, Cell Death & Disease, and Molecular Cell. Associated research fields include oxidative stress and redox biology, neurodegeneration (ALS), ferroptosis, cardiovascular disease, renal fibrosis, cancer, diabetes, mitochondrial biology, autophagy, inflammation, and tissue repair/regeneration.
| Species | Application | Title |
|---|---|---|
Bioact Mater A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant | ||
Bioact Mater Dynamic degradation patterns of porous polycaprolactone/β-tricalcium phosphate composites orchestrate macrophage responses and immunoregulatory bone regeneration | ||
Cell Stem Cell Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons | ||
Cancer Res GFER Represents a Target for Dual Disruption of Redox Homeostasis and Reactivation of the Immune Response in Pancreatic Adenocarcinoma.
| ||
Autophagy Deficiency of mitophagy receptor FUNDC1 impairs mitochondrial quality and aggravates dietary-induced obesity and metabolic syndrome. |
Reviews
What customers say
Users report consistently positive results with this SOD1 antibody across Western blot, immunofluorescence, and immunohistochemistry. Seven of eight reviewers rated it 5 stars. Western blot users observed clear bands at the expected molecular weight in mouse brain, kidney tissue, and 293T cells at dilutions ranging from 1:1000 to 1:15000. Immunofluorescence on ovarian tissue and immunocytochemistry on NSC-34 cells both showed strong labeling. One reviewer gave 3 stars but confirmed it worked fine in Western blot. Overall, the antibody is well-regarded for specificity and performance across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Georgia (Verified Customer) (11-27-2025) | Corpus callosum mouse brain lysates incubated with SOD1 antibody at 1:1000 for 2 hours at RT
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Prakash (Verified Customer) (10-28-2025) | work fine in western blot
|
Scott (Verified Customer) (10-22-2024) | 20µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size. Precision plus protein standard ladder #1610373. First 5 lanes are Transgenic SOD1G93A mice, last four lanes are non-transgenic mice.
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Tianyi (Verified Customer) (08-17-2023) | Paraffin-embedded tissues (4% PFA, 24 h) incubated with SOD1 (1:200) and secondary antibody Alexa Fluor donkey anti-rabbit 594 IgG (H+L).
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Xingyuan (Verified Customer) (07-17-2023) | Quality was decent.
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Macarena Lucia (Verified Customer) (10-17-2022) | clear band.
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Elise (Verified Customer) (06-24-2022) | Tested on Western blot for 20µg of protein from kidney tissue. Antibody diluted at 1 :1000 in TBS-T BSA 5 %
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Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.
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