SOD2 Polyclonal antibody

SOD2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24127-1-AP

Synonyms

IPO B, Mn SOD, MNSOD, SOD2, SODM



Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24127-1-AP targets SOD2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, sheep, pig, Bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOD2 fusion protein Ag21388
Full Name superoxide dismutase 2, mitochondrial
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC016934
Gene Symbol SOD2
Gene ID (NCBI) 6648
RRIDAB_2879437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Superoxide dismutase 2 (SOD2), also known as MnSOD (manganese-dependent superoxide dismutase), is a 

mitochondrial enzyme that dismutates superoxide ions.


What is the molecular weight of SOD2? Is SOD2 post-translationally modified?

The molecular weight of SOD2 is 25 kDa. SOD2 acts as a homotetramer with manganese ion. SOD2 can be 

post-translationally modified, including phosphorylation, nitration, and acetylation. Phosphorylation of SOD2 

increases its enzymatic activity and occurs as part of the adaptive response to radiation stress (PMID: 23243068), 

while nitration and acetylation decrease SOD2 enzymatic activity (PMID: 20106845, 21172655).


What is the subcellular localization of superoxide dismutases?

SOD2 is the only superoxide dismutase that localizes to the mitochondrial matrix (PMID: 4578091). SOD1 is 

predominantly found in the cytoplasm and to a lesser extent in the mitochondrial intermembrane space and 

nucleus (PMID: 11507097), while SOD3 localizes extracellularly.


What is the role of SOD2 in neutralizing reactive oxygen species (ROS)?

Superoxide is a main form of ROS that originates directly from the electron transport chain in the mitochondrial 

matrix. SOD2 plays a crucial role in mitochondrial ROS homeostasis by catalyzing turnover of superoxide radicals 

to hydrogen peroxide and molecular oxygen. ROS are potent physiological signaling molecules but at high doses 

they contribute to the pathogenesis of many diseases, including cardiovascular and neurodegenerative diseases 

and cancer (PMID: 29669742). SOD2 also associates with mitochondrial DNA (mtDNA), protecting it from 

oxidative damage (PMID: 19228881).


What is the tissue expression pattern of SOD2?

SOD2 is ubiquitously expressed.


Can SOD2 be used as a marker of oxidative stress?

SOD2 is a key player in antioxidant defense and contributes to the regulation of oxidative stress. Defects in SOD2 

activity or levels can result in oxidative stress. However, SOD2 cannot be used as a marker of oxidative stress 

because its expression is not always altered by oxygen stress. 


Protocols

Product Specific Protocols
WB protocol for SOD2 antibody 24127-1-APDownload protocol
IHC protocol for SOD2 antibody 24127-1-APDownload protocol
IF protocol for SOD2 antibody 24127-1-APDownload protocol
IP protocol for SOD2 antibody 24127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Authors - Calvin S Carter
mouse,humanWB

ACS Nano

Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.

Authors - Meng Zhao
humanWB

Adv Sci (Weinh)

IGFBP1 Sustains Cell Survival during Spatially-Confined Migration and Promotes Tumor Metastasis

Authors - Guoqing Cai
humanWB

Nat Commun

Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.

Authors - Haohao Huang
mouseWB

Redox Biol

miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation

Authors - Xiaofei Wang
mouseWB

Nat Commun

The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.

Authors - Guang Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000