- Featured Product
- KD/KO Validated
SOX5 Polyclonal antibody
SOX5 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13216-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, SH-SY5Y cells, U-87 MG cells, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
13216-1-AP targets SOX5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SOX5 fusion protein Ag4092 |
| Full Name | SRY (sex determining region Y)-box 5 |
| Calculated Molecular Weight | 763 aa, 84 kDa |
| Observed Molecular Weight | 68-70 kDa |
| GenBank Accession Number | BC029220 |
| Gene Symbol | SOX5 |
| Gene ID (NCBI) | 6660 |
| RRID | AB_2196089 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sex determining region Y-box protein 5 (SOX5) is a member of the SOX family, which was identified based on the conserved homology of the high-mobility group DNA-binding motif. It has been reported that SOX5 is involved in the regulation of embryonic development, and is associated with various cancer types, including prostate cancer, glioblastoma, hepatocellular carcinoma, osteosarcoma and nasopharyngeal carcinoma. SOX5 has 5 isoforms with the molecular mass of 42, 71 and 82-84 kDa. (PMID: 30854049)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX5 antibody 13216-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination. | ||
Pharmacol Res Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways. | ||
Cell Oncol (Dordr) Apatinib inhibits glycolysis by suppressing the VEGFR2/AKT1/SOX5/GLUT4 signaling pathway in ovarian cancer cells.
| ||
Phytomedicine Transcriptomics, molecular docking, and cross-resistance profiling of nobiletin in cancer cells and synergistic interaction with doxorubicin upon SOX5 transfection.
| ||
Lab Invest Sox13 promotes hepatocellular carcinoma metastasis by transcriptionally activating Twist1. | ||
Mol Cell Biochem Expression profiles of Sox transcription factors within the postnatal rodent testes. |
