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SRA1 Polyclonal antibody

SRA1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 18699-1-AP

Synonyms

pp7684, SRA, SRA1, SRAP, STRAA1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using SRA1 Polyclonal antibody (18699-1-AP)

    WB analysis of A431 using 18699-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 18699-1-AP (SRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SRA1 Polyclonal antibody (18699-1-AP)

    WB analysis of MCF-7 using 18699-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18699-1-AP (SRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SRA1 Polyclonal antibody (18699-1-AP)

    IF Staining of A431 using 18699-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 18699-1-AP (SRA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18699-1-AP targets SRA1 in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name steroid receptor RNA activator 1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC156760
Gene Symbol SRA1
Gene ID (NCBI) 10011
RRIDAB_2270887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription.

Protocols

Product Specific Protocols
WB protocol for SRA1 antibody 18699-1-APDownload protocol
IF protocol for SRA1 antibody 18699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols