SRC Antibody

SRC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 11097-1-AP

Synonyms

ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1



Tested Applications

Positive WB detected inJurkat cells, A431 cells, CHO cells, COLO 320 cells, mouse brain tissue, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman breast cancer tissue, human brain tissue, human spleen tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:800
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11097-1-AP targets SRC in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Reactivity human, mouse, rat, hamster
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRC fusion protein Ag1575
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated molecular weight 60 kDa
Observed molecular weight 55 kDa, 60 kDa
GenBank accession number BC011566
Gene symbol SRC
Gene ID (NCBI) 6714
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).

Protocols

Product Specific Protocols
WB protocol for SRC antibody 11097-1-APDownload protocol
IHC protocol for SRC antibody 11097-1-APDownload protocol
IF protocol for SRC antibody 11097-1-APDownload protocol
IP protocol for SRC antibody 11097-1-APDownload protocol
FC protocol for SRC antibody 11097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Aging (Albany NY)

DR region specific antibody ameliorated but ouabain worsened renal injury in nephrectomized rats through regulating Na,K-ATPase mediated signaling pathways.

Authors - Juan Wang
humanWB

Front Oncol

SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.

Authors - Ruoyan Liu
humanWB

Sci Adv

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Authors - Patrick Han
humanIHC

Ann Otol Rhinol Laryngol

Hypoxia-Inducible Factor-1α (HIF-1α) Expression on Endothelial Cells in Juvenile Nasopharyngeal Angiofibroma: A Review of 70 cases and Tissue Microarray Analysis.

Authors - Xiaole Song
mouseIHC

J Mol Histol

Histochemical evidence of zoledronate inhibiting c-src expression and interfering with CD44/OPN-mediated osteoclast adhesion in the tibiae of mice.

Authors - Hongrui Liu
humanIHC

Neuropathology

Phosphorylated Syk expression is enhanced in Nasu-Hakola disease brains.

Authors - Satoh Jun-Ichi J