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SSPN Polyclonal antibody

SSPN Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 26685-1-AP

Synonyms

DAGA5, Kirsten ras associated protein, KRAG, nanospan, NSPN, Sarcospan, SPN1, SPN2, SSPN

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SSPN Polyclonal antibody (26685-1-AP)

    WB analysis of mouse liver using 26685-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26685-1-AP (SSPN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using SSPN Polyclonal antibody (26685-1-AP)

    WB analysis of rat liver using 26685-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 26685-1-AP (SSPN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26685-1-AP is SSPN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSPN fusion protein Ag24511
Full Name sarcospan (Kras oncogene-associated gene)
Calculated Molecular Weight 243 aa, 27 kDa
Observed Molecular Weight 15-20 kDa, 27 kDa
GenBank Accession NumberBC062299
Gene Symbol SSPN
Gene ID (NCBI) 8082
RRIDAB_2880602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SSPN antibody 26685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols