SSTR3 Polyclonal antibody

SSTR3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20696-1-AP

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

CD377, somatostatin receptor 3, Somatostatin receptor type 3, SS 3 R, SS3 R

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of fetal human brain using 20696-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20696-1-AP (SSTR3 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pituitary using 20696-1-AP
Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 20696-1-AP (SSTR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pituitary using 20696-1-AP
Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 20696-1-AP (SSTR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 20696-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20696-1-AP (SSTR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 20696-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20696-1-AP (SSTR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 20696-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20696-1-AP (SSTR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 20696-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20696-1-AP (SSTR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	fetal human brain tissue
Positive IHC detected in	human pituitary tissue, human gliomas tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20696-1-AP targets SSTR3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	somatostatin receptor 3
Calculated Molecular Weight	46 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	NM_001051
Gene Symbol	SSTR3
Gene ID (NCBI)	6753
RRID	AB_2878725
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P32745
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SSTR3 antibody 20696-1-AP	Download protocol
WB protocol for SSTR3 antibody 20696-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The SSTR3 Polyclonal antibody (Cat# 20696-1-AP) has 4 total citations. These appear in EMBO Reports, Molecular Biology of the Cell, Journal of Histochemistry & Cytochemistry, and Cell Communication and Signaling. The cited research spans ciliary assembly and signaling, ciliary gating at the vertebrate transition zone, immunohistochemical profiling of somatostatin receptor subtypes in neuroendocrine neoplasias, and AKAP2/PKA-mediated ciliogenesis and Shh signaling. Cited applications include IF and IHC in human samples.

Species	Application	Title
human	IF	EMBO Rep Ubiquitylation of BBSome is required for ciliary assembly and signaling Authors - Francesco Chiuso View Article
human	IF	Mol Biol Cell Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone. Authors - Antonia Wiegering View Article
human	IHC	J Histochem Cytochem Immunohistochemical Expression of Somatostatin Receptor Subtypes in a Panel of Neuroendocrine Neoplasias. Authors - Satu M Remes View Article
		Cell Commun Signal AKAP2/PKA complex coordinates primary ciliogenesis, ciliary trafficking and Shh signaling. Authors - Francesco Chiuso View Article