STAT4 Antibody

STAT4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 51070-2-AP

Synonyms

SLEB11, STAT4



Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman cervical cancer tissue, human testis tissue, rat testis tissue, mouse testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

51070-2-AP targets STAT4 in IF, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 4
Calculated molecular weight 748aa,86 kDa
Observed molecular weight 86 kDa
GenBank accession number BC031212
Gene symbol STAT4
Gene ID (NCBI) 6775
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].

Protocols

Product Specific Protocols
WB protocol for STAT4 antibody 51070-2-APDownload protocol
IHC protocol for STAT4 antibody 51070-2-APDownload protocol
IF protocol for STAT4 antibody 51070-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Immunol

Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells.

Authors - James P Hewitson