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STAT5A-Specific Polyclonal antibody

STAT5A-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51074-2-AP

Synonyms

MGF, STAT5, STAT5A, STAT5A-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    WB analysis of NIH/3T3 using 51074-2-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51074-2-AP (STAT5A-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    WB analysis of HeLa using 51074-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51074-2-AP (STAT5A-specific antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    WB analysis of HepG2 using 51074-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 51074-2-AP (STAT5A-specific antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human cervical cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51074-2-AP (STAT5A-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human cervical cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51074-2-AP (STAT5A-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human breast cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51074-2-AP (STAT5A-specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human breast cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51074-2-AP (STAT5A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human lung cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51074-2-AP (STAT5A-specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human lung cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51074-2-AP (STAT5A Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51074-2-AP targets STAT5A-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 5A
Calculated Molecular Weight 794 aa, 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
RRIDAB_2196758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GH. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is specific to STAT5A. It has no cross reaction to STAT5B.

Protocols

Product Specific Protocols
WB protocol for STAT5A-Specific antibody 51074-2-APDownload protocol
IHC protocol for STAT5A-Specific antibody 51074-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols