STAT5A Polyclonal antibody

STAT5A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51074-2-AP

Synonyms

MGF, STAT5, STAT5A, STAT5A-Specific



Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

51074-2-AP targets STAT5A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 5A
Calculated molecular weight 794 aa, 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GH. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is specific to STAT5A. It has no cross reaction to STAT5B.

Protocols

Product Specific Protocols
WB protocol for STAT5A antibody 51074-2-APDownload protocol
IHC protocol for STAT5A antibody 51074-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols