STAT5B Polyclonal antibody

STAT5B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12071-1-AP

Synonyms

STAT5, STAT5B



Tested Applications

Positive WB detected inHeLa cells, multi-cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12071-1-AP targets STAT5B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAT5B fusion protein Ag2709
Full Name signal transducer and activator of transcription 5B
Calculated molecular weight 787 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC020868
Gene symbol STAT5B
Gene ID (NCBI) 6777
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT5B.

Protocols

Product Specific Protocols
WB protocol for STAT5B antibody 12071-1-APDownload protocol
IHC protocol for STAT5B antibody 12071-1-APDownload protocol
IF protocol for STAT5B antibody 12071-1-APDownload protocol
IP protocol for STAT5B antibody 12071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB, IP, IF

Toxicol Appl Pharmacol

PMID: 26493931

Authors - Furong Zhang
ratWB

Biomed Pharmacother

Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats.

Authors - Yu Long
mouseWB

Biochem Biophys Res Commun

1, 25(OH)2D3-induced interaction of vitamin D receptor with p50 subunit of NF-κB suppresses the interaction between KLF5 and p50, contributing to inhibition of LPS-induced macrophage proliferation.

Authors - Dong Ma