Stomatin Antibody

Stomatin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 12046-1-AP

Synonyms

BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin



Tested Applications

Positive WB detected inHeLa cells, A549 cells, human liver tissue, human lung tissue, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12046-1-AP targets Stomatin in IF, IHC, IP, WB, ELISA applications and shows reactivity with human samples.

Reactivity human
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Stomatin fusion protein Ag2676
Full Name stomatin
Calculated molecular weight 288aa,32 kDa
Observed molecular weight 32 kDa
GenBank accession number BC010703
Gene symbol STOM
Gene ID (NCBI) 2040
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

Protocols

Product Specific Protocols
WB protocol for Stomatin antibody 12046-1-APDownload protocol
IHC protocol for Stomatin antibody 12046-1-APDownload protocol
IF protocol for Stomatin antibody 12046-1-APDownload protocol
IP protocol for Stomatin antibody 12046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Chin J Cancer Res

Stomatin plays a suppressor role in non-small cell lung cancer metastasis.

Authors - Huaying An
humanWB

Vox Sang

Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools.

Authors - Rubin O O