• Featured Product
  • KD/KO Validated

STX17 Polyclonal antibody

STX17 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17815-1-AP

Synonyms

Syntaxin-17



Tested Applications

Positive WB detected inA375 cells, HepG2 cells, human testis tissue, L02 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17815-1-AP targets STX17 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STX17 fusion protein Ag12134
Full Name syntaxin 17
Calculated Molecular Weight 302 aa, 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC111009
Gene Symbol STX17
Gene ID (NCBI) 55014
RRIDAB_2255542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC, and the Golgi.

Protocols

Product Specific Protocols
WB protocol for STX17 antibody 17815-1-APDownload protocol
IHC protocol for STX17 antibody 17815-1-APDownload protocol
IP protocol for STX17 antibody 17815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis.

Authors - Hua Su
humanWB,IF,IP

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
humanWB

Autophagy

DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.

Authors - Xiaoyu Tian
mouseWB

Autophagy

PMID: 32432943

Authors - Zu-Guo Zheng
  • KD Validated
humanWB

Autophagy

Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

Authors - Jia-min Yan
  • KO Validated
humanWB

Autophagy

Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.

Authors - Xu Li
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jane (Verified Customer) (01-07-2022)

No working, non specific bands.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Heart/Cardiomyocytes
STX17 Antibody Western Blot,Immunohistochemistry validation (1:500 dilution) in Heart/Cardiomyocytes (Cat no:17815-1-AP)