Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue, HEK-293 cells, Jurkat cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14988-1-AP targets Syntaxin 4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6973 Product name: Recombinant human STX4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC002436 Sequence: MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKK Predict reactive species |
| Full Name | syntaxin 4 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 35-37 kDa |
| GenBank Accession Number | BC002436 |
| Gene Symbol | STX4 |
| Gene ID (NCBI) | 6810 |
| RRID | AB_2286910 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12846 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Syntaxin 4 antibody 14988-1-AP | Download protocol |
| IP protocol for Syntaxin 4 antibody 14988-1-AP | Download protocol |
| WB protocol for Syntaxin 4 antibody 14988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Sec22b (Cat# 14988-1-AP) has 12 citations spanning journals including Nature Metabolism, EMBO Journal, Brain, JBC, Frontiers in Immunology, iScience, Neurochemistry International, Journal of Translational Medicine, British Journal of Pharmacology, International Journal of Biological Macromolecules, Neuroscience, and Cell Structure and Function. Research fields cover SNARE-mediated exosome secretion, liver glycogen metabolism, lysosomal exocytosis, atherosclerosis, cytotoxic lymphocyte killing, monocyte migration, inflammatory pain, and pathogen-host SNARE interactions.
| Species | Application | Title |
|---|---|---|
Brain Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction. | ||
J Transl Med Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure. | ||
Int J Biol Macromol PKCε controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode. | ||
Br J Pharmacol CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells. |
