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SULT1A1 Monoclonal antibody

SULT1A1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

10H6F6

Cat no : 60076-1-Ig

Synonyms

Aryl sulfotransferase 1, HAST1/HAST2, P PST, P PST 1, Phenol sulfotransferase 1, PST, ST1A1, ST1A3, STP, STP1, Sulfotransferase 1A1, SULT1A1, Ts PST, TSPST1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SULT1A1 Monoclonal antibody (60076-1-Ig)

    WB analysis using 60076-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SULT1A1 Monoclonal antibody (60076-1-Ig)

    WB analysis of Jurkat using 60076-1-Ig

    WB result of SULT1A1 antibody (60076-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SULT1A1 transfected Jurkat cells.

  • Western Blot (WB) analysis of human placenta tissue using SULT1A1 Monoclonal antibody (60076-1-Ig)

    WB analysis of human placenta using 60076-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SULT1A1 Monoclonal antibody (60076-1-Ig)

    WB analysis of human liver using 60076-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SULT1A1 Monoclonal antibody (60076-1-Ig)

    WB analysis using 60076-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig brain tissue, U2OS cells, human placenta tissue, human liver tissue, Jurkat cells, HepG2 cells, HEK-293 cells, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60076-1-Ig targets SULT1A1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SULT1A1 fusion protein Ag4611
Full Name sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000923
Gene Symbol SULT1A1
Gene ID (NCBI) 6817
RRIDAB_2197592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT1A1, also named as ST1A1, P-PST 1, ST1A3, Ts-PST, P-PST 1, STP and STP1, belongs to the sulfotransferase 1 family. It catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. SULT1A1 is also responsible for the sulfation and activation of minoxidil. It mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity.

Protocols

Product Specific Protocols
WB protocol for SULT1A1 antibody 60076-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols