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SULT1A4 Polyclonal antibody

SULT1A4 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11376-1-AP

Synonyms

Aryl sulfotransferase 1A3/1A4, HAST3, M PST, ST1A3/ST1A4, STM, Sulfotransferase 1A3/1A4, SULT1A3, SULT1A4, TL PST

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using SULT1A4 Polyclonal antibody (11376-1-AP)

    WB analysis of human colon using 11376-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human pancreas tissue using SULT1A4 Polyclonal antibody (11376-1-AP)

    WB analysis of human pancreas using 11376-1-AP

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using SULT1A4 Polyclonal antibody (11376-1-AP)

    WB analysis of mouse pancreas using 11376-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using SULT1A4 Polyclonal antibody (11376-1-AP)

    WB analysis of BxPC-3 using 11376-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SULT1A4 Polyclonal antibody (11376-1-AP)

    WB analysis of HepG2 using 11376-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A4 Polyclonal antibody (11376-1-AP)

    IHC staining of human breast cancer using 11376-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11376-1-AP (SULT1A4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A4 Polyclonal antibody (11376-1-AP)

    IHC staining of human breast cancer using 11376-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11376-1-AP (SULT1A4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SULT1A4 Polyclonal antibody (11376-1-AP)

    IF Staining of HepG2 using 11376-1-AP

    Immunofluorescent analysis of HepG2 cells using 11376-1-AP (SULT1A4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SULT1A4 Polyclonal antibody (11376-1-AP)

    IF Staining of HepG2 using 11376-1-AP

    Immunofluorescent analysis of HepG2 cells, using SULT1A4 antibody 11376-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman colon tissue, BxPC-3 cells, HepG2 cells, human pancreas tissue, mouse pancreas tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11376-1-AP targets SULT1A4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SULT1A4 fusion protein Ag1935
Full Name sulfotransferase family, cytosolic, 1A, phenol-preferring, member 4
Calculated Molecular Weight 295 aa, 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC014471
Gene Symbol SULT1A4
Gene ID (NCBI) 445329
RRIDAB_2197610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SULT1A4 antibody 11376-1-APDownload protocol
IHC protocol for SULT1A4 antibody 11376-1-APDownload protocol
IF protocol for SULT1A4 antibody 11376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols