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TAF12 Polyclonal antibody

TAF12 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 12353-1-AP

Synonyms

TAF12, TAF15, TAF2J, TAFII 20/TAFII 15, TAFII20, TAFII20/TAFII15



Tested Applications

Positive WB detected inY79 cells
Positive IP detected inY79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12353-1-AP targets TAF12 in WB, IP, ChIP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAF12 fusion protein Ag3017
Full Name TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
Calculated molecular weight 18 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC011986
Gene symbol TAF12
Gene ID (NCBI) 6883
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation. Observed MW of TAF12 is from 14-17 kDa and 17-21 kDa.

Protocols

Product Specific Protocols
WB protocol for TAF12 antibody 12353-1-APDownload protocol
IP protocol for TAF12 antibody 12353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Biol

The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells.

Authors - Gegonne Anne A
humanWB

Mol Cell Biol

The double-histone-acetyltransferase complex ATAC is essential for mammalian development.

Authors - Guelman Sebastián S
mouseChIP,WB

J Bone Miner Res

Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease.

Authors - Jumpei Teramachi
mouse, humanWB

J Bone Miner Res

TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation.

Authors - Sun Quanhong Q
mouseWB

J Bone Miner Res

Increased IL-6 expression in osteoclasts is necessary but not sufficient for the development of Paget's disease of bone.

Authors - Jumpei Teramachi
mouseWB

Cell Metab

Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease.

Authors - Noriyoshi Kurihara