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  • KD/KO Validated

Transgelin 2 Polyclonal antibody

Transgelin 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10234-2-AP

Synonyms

SM22 beta, SM22beta, TAGLN2, Transgelin 2



Tested Applications

Positive WB detected inhuman heart tissue, HEK-293 cells, T-47D cells
Positive IHC detected inhuman colon cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10234-2-AP targets Transgelin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Transgelin 2 fusion protein Ag0337
Full Name transgelin 2
Calculated molecular weight 199 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC002616
Gene symbol TAGLN2
Gene ID (NCBI) 8407
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 2 protein. It may cross react with other two transgelins based on the sequence similarity.

Protocols

Product Specific Protocols
WB protocol for Transgelin 2 antibody 10234-2-APDownload protocol
IHC protocol for Transgelin 2 antibody 10234-2-APDownload protocol
IF protocol for Transgelin 2 antibody 10234-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

FASEB J

Cytoskeletal transgelin 2 contributes to gender-dependent adipose tissue expandability and immune function.

Authors - Francisco J Ortega
  • KD Validated
humanWB

J Obstet Gynaecol Res

Functional significance of transgelin-2 in uterine cervical squamous cell carcinoma.

Authors - Kazuyuki Yakabe
  • KD Validated
humanWB

Oncol Lett

iTRAQ-coupled 2D LC/MS-MS analysis of CXCR7-transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers.

Authors - Hengwei Zhang
humanIHC

J Vasc Interv Radiol

Usefulness of material recovered from distal embolic protection devices after carotid angioplasty for proteomic studies.

Authors - Cristobo Iván I

Biochem Biophys Res Commun

TAGLN2, a novel regulator involved in Hepatitis B virus transcription and replication.

Authors - Youjia Yu
mouseWB

Mol Immunol

B-1 cells express transgelin 2: unexpected lymphocyte expression of a smooth muscle protein identified by proteomic analysis of peritoneal B-1 cells.

Authors - Francés Rubén R

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-16-2019)

It recognizes transgelin (prominent lower band) and some other bands, albeit much more weakly.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Transgelin 2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10234-2-AP)