|Positive WB detected in||human heart tissue, HEK-293 cells, T-47D cells|
|Positive IHC detected in||human colon cancer tissue, human urothelial carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10234-2-AP targets Transgelin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Transgelin 2 fusion protein Ag0337|
|Full Name||transgelin 2|
|Calculated molecular weight||199 aa, 22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC002616|
|Gene ID (NCBI)||8407|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 2 protein. It may cross react with other two transgelins based on the sequence similarity.
Cytoskeletal transgelin 2 contributes to gender-dependent adipose tissue expandability and immune function.
J Obstet Gynaecol Res
Functional significance of transgelin-2 in uterine cervical squamous cell carcinoma.
iTRAQ-coupled 2D LC/MS-MS analysis of CXCR7-transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers.
J Vasc Interv Radiol
Usefulness of material recovered from distal embolic protection devices after carotid angioplasty for proteomic studies.
Biochem Biophys Res Commun
TAGLN2, a novel regulator involved in Hepatitis B virus transcription and replication.
B-1 cells express transgelin 2: unexpected lymphocyte expression of a smooth muscle protein identified by proteomic analysis of peritoneal B-1 cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-16-2019)
It recognizes transgelin (prominent lower band) and some other bands, albeit much more weakly.