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TAU Monoclonal antibody

TAU Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E9A8

Cat no : 66499-1-Ig

Synonyms

DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU



Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, U-251 cells, Y79 cells, pig brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:30000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66499-1-Ig targets TAU in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivity human, rat
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen TAU fusion protein Ag21926
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000558
Gene symbol MAPT
Gene ID (NCBI) 4137
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability.

Protocols

Product Specific Protocols
WB protocol for TAU antibody 66499-1-IgDownload protocol
IHC protocol for TAU antibody 66499-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Environ Toxicol

Cadmium induces actin cytoskeleton alterations and dysfunction in Neuro-2a cells.

Authors - Yaming Ge
ratWB

J Pineal Res

PMID: 31050371

Authors - Jiqu Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Virender (Verified Customer) (01-09-2020)

Tau antibody works well for recombinant tau as well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
FH

Mary (Verified Customer) (11-08-2019)

I like this antibody. It provides a clear specific signal. The shipping was prompt and the antibody was kept on ice the whole time which I appreciated.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y