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TBC1D15 Polyclonal antibody

TBC1D15 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17252-1-AP

Synonyms

GAP for RAB7, GTPase activating protein RAB7, Rab7 GAP, TBC1 domain family member 15, TBC1 domain family, member 15, TBC1D15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D15 Polyclonal antibody (17252-1-AP)

    WB analysis using 17252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TBC1D15 Polyclonal antibody (17252-1-AP)

    WB analysis using 17252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBC1D15 Polyclonal antibody (17252-1-AP)

    IF Staining of HeLa using 17252-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TBC1D15 antibody (17252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, A431 cells, mouse liver tissue, rat liver tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17252-1-AP targets TBC1D15 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D15 fusion protein Ag10625
Full Name TBC1 domain family, member 15
Calculated Molecular Weight 691 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC028352
Gene Symbol TBC1D15
Gene ID (NCBI) 64786
RRIDAB_2878370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D15 is a highly conserved protein belonging to the TBC domain family and has similar functions to Rab-GAP. TBC1D15 is ubiquitously expressed and localized predominantly to the cytosol. TBC1D15 is a protein that contains a conserved Tre2/Bub2/Cdc16 (TBC) domain. TBC1D15 can serve as a mitochondrial Rab GTPase-activating protein (Rab-GAP) and influence autophagosome biogenesis and morphology downstream of Parkin activation. TBC1D15 can also control stem cell self-renewal when coupled with the Numb-p53 complex.

Protocols

Product Specific Protocols
WB protocol for TBC1D15 antibody 17252-1-APDownload protocol
IF protocol for TBC1D15 antibody 17252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols