Tested Applications
| Positive WB detected in | mouse liver tissue, mouse brain tissue, Jurkat cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10502-1-AP targets TCEB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0778 Product name: Recombinant human TCEB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC002883 Sequence: MAAESALQVVEKLQARLAANPDPKKLLKYLKKLSTLPITVDILAETGVGKTVNSLRKHEHVGSFARDLVAQWKKLVPVERNAEPDEQDFEKSNSRKRPRDALQKEEEMEGDYQETWKATGSRSYSPDHRQKKHRKLSELERPHKVSHGHERRDERKRCHRMSPTYSSDPESSDYGHVQSPPSCTSPHQMYVDHYRSLEEDQEPIVSHQKPGKGHSNAFQDRLGASQERHLGEPHGKGVVSQNKEHKSSHKDKRPVDAKSDEKASVVSREKSHKALSKEENRRPPSGDNAREKPPSSGVKKEKDREGSSLKKKCLPPSEAASDNHLKKPKHRDPEKAKLDKSKQGLDSFDTGKGAGDLLPKVKEKGSNNLKTPEGKVKTNLDRKSLGSLPKVEETDMEDEF Predict reactive species |
| Full Name | transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) |
| Calculated Molecular Weight | 110 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC002883 |
| Gene Symbol | TCEB3 |
| Gene ID (NCBI) | 6924 |
| RRID | AB_2201278 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14241 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TCEB3 antibody 10502-1-AP | Download protocol |
| IHC protocol for TCEB3 antibody 10502-1-AP | Download protocol |
| WB protocol for TCEB3 antibody 10502-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TCEB3 Polyclonal antibody (Cat# 10502-1-AP) has 1 total citation. It was cited in FASEB Journal in a 2024 study investigating TCEB3-mediated ubiquitination and degradation of MCL-1 in ovarian cancer apoptosis. The research field encompasses cell death (apoptosis), protein modification (ubiquitination), and oncology (ovarian cancer). The antibody was validated by RNAi knockdown and applied in Western blot for human samples.
| Species | Application | Title |
|---|---|---|
FASEB J TCEB3 initiates ovarian cancer apoptosis by mediating ubiquitination and degradation of MCL-1
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