Tested Applications
| Positive WB detected in | MG-63 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86294-2-RR targets TCF21 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag32510 Product name: Recombinant human TCF21 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-80 aa of BC025697 Sequence: LSDVEDLQEVEMLECDGLKMDSNKEFVTSNESTEESSNCENGSPQKGRGGLGKRRKAPTKKSPLSGVSQEGKQVQ Predict reactive species |
| Full Name | transcription factor 21 |
| Calculated Molecular Weight | 179 aa, 20 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC025697 |
| Gene Symbol | TCF21 |
| Gene ID (NCBI) | 6943 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O43680 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TCF21 (Transcription factor 21), also known as POD1. It is predicted to be located in the nucleus. TCF21 is a prognostic marker in Breast invasive carcinoma, Kidney renal clear cell carcinoma, Liver hepatocellular carcinoma, Lung squamous cell carcinoma. This protein is involved in the epithelial-mesenchymal interaction in the morphogenesis of kidney and lung, including epithelial differentiation and branching morphogenesis. It may play a role in the specialization or differentiation of one or more epicardial cell types. The molecular weight of TCF21 is 20 kDa, it also has phosphorylation modification.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TCF21 antibody 86294-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
