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KD/KO Validated

TCF3 Polyclonal antibody

Name: TCF3 Polyclonal antibody
Brand: Proteintech
SKU: 21242-1-AP

TCF3 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21242-1-AP

Synonyms

bHLHb21, E2A, E47, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1

Validation Data Gallery

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AllWBIPIF

WB analysis of Jurkat using 21242-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 21242-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 21242-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21242-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 21242-1-AP
IP result of anti-TCF3 (IP:21242-1-AP, 4ug; Detection:21242-1-AP 1:500) with Jurkat cells lysate 2800ug.
IF Staining of HepG2 using 21242-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21242-1-AP (E2A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 21242-1-AP

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Tested Applications

Positive WB detected in	Jurkat cells, PC-3 cells, K-562 cells, NIH/3T3 cells
Positive IP detected in	Jurkat cells
Positive IF detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

21242-1-AP targets TCF3 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TCF3 fusion protein Ag15734
Full Name	transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Calculated Molecular Weight	654 aa, 68 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC110580
Gene Symbol	TCF3
Gene ID (NCBI)	6929
RRID	AB_10755295
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TCF3 antibody 21242-1-AP	Download protocol
IF protocol for TCF3 antibody 21242-1-AP	Download protocol
IP protocol for TCF3 antibody 21242-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Cell Signal Targeting SDCBP2 in acute myeloid leukemia Authors - Yan Du View Article
human	WB	J Cell Mol Med The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells. Authors - Meiju Zhang KD Validated View Article
mouse	WB	Mol Immunol Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development. Authors - Wei Liang View Article
rat	IP	Life Sci miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling. Authors - Ting Liu View Article
mouse	WB	Nat Commun A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation Authors - Jianfeng Wu View Article

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TCF3 Polyclonal antibody

TCF3 Polyclonal Antibody for IF, IP, WB, ELISA

Validation Data Gallery

WB analysis of Jurkat using 21242-1-AP

WB analysis using 21242-1-AP

WB analysis of PC-3 using 21242-1-AP

WB analysis of K-562 using 21242-1-AP

IP experiment of Jurkat using 21242-1-AP

IF Staining of HepG2 using 21242-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Targeting SDCBP2 in acute myeloid leukemia

The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells.

Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development.

miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling.

A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation