E2A Polyclonal antibody

E2A Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 21242-1-AP

Synonyms

bHLHb21, E2A, E47, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, PC-3 cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21242-1-AP is E2A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E2A fusion protein Ag15734
Full Name transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Calculated molecular weight 654 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC110580
Gene symbol TCF3
Gene ID (NCBI) 6929
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for E2A antibody 21242-1-APDownload protocol
IF protocol for E2A antibody 21242-1-APDownload protocol
IP protocol for E2A antibody 21242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIP

Life Sci

miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling.

Authors - Ting Liu
mouseWB

Mol Immunol

Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development.

Authors - Wei Liang