HIC5 Polyclonal antibody

HIC5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10565-1-AP

Synonyms

ARA55, HIC 5, HIC5, TGFB1I1, TSC 5



Tested Applications

Positive WB detected inmouse colon tissue, A431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10565-1-AP targets HIC5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIC5 fusion protein Ag0868
Full Name transforming growth factor beta 1 induced transcript 1
Calculated molecular weight 48 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC001830
Gene symbol TGFB1I1
Gene ID (NCBI) 7041
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HIC5 antibody 10565-1-APDownload protocol
IHC protocol for HIC5 antibody 10565-1-APDownload protocol
IF protocol for HIC5 antibody 10565-1-APDownload protocol
IP protocol for HIC5 antibody 10565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Cell Death Dis

HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression.

Authors - Xuanling Du
humanWB

J Proteome Res

Quantitative proteomics implicates Rictor/mTORC2 in cell adhesion.

Authors - Hao Wang