TGF Beta 3 Polyclonal antibody
TGF Beta 3 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 18942-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | 3T3-L1 cells, mouse ovary tissue, HeLa cells, PC-12 cells |
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse ovary tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 12 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
18942-1-AP targets TGF Beta 3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TGF Beta 3 fusion protein Ag13507 |
| Full Name | transforming growth factor, beta 3 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC018503 |
| Gene Symbol | TGFB3 |
| Gene ID (NCBI) | 7043 |
| RRID | AB_2202206 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TGF Beta 3 antibody 18942-1-AP | Download protocol |
| IHC protocol for TGF Beta 3 antibody 18942-1-AP | Download protocol |
| IF protocol for TGF Beta 3 antibody 18942-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res SIX1 promotes tumor lymphangiogenesis by coordinating TGFβ signals that increase expression of VEGF-C. | ||
NPJ Regen Med Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration. | ||
J Med Chem Dithiocarbazate-Copper Complexes for Bioimaging and Treatment of Pancreatic Cancer. | ||
Sci Rep Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling. | ||
Sci Rep Transforming growth factor beta 3 involved in the pathogenesis of synovial chondromatosis of temporomandibular joint. |
