TIMM17B Polyclonal antibody

TIMM17B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11062-1-AP

Synonyms

DXS9822, JM3, TIM17B, TIMM17B



Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

11062-1-AP targets TIMM17B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMM17B fusion protein Ag1504
Full Name translocase of inner mitochondrial membrane 17 homolog B (yeast)
Calculated molecular weight 18 kDa
Observed molecular weight 17-20 kDa
GenBank accession numberBC010142
Gene symbol TIMM17B
Gene ID (NCBI) 10245
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TIMM17B antibody 11062-1-APDownload protocol
IHC protocol for TIMM17B antibody 11062-1-APDownload protocol
IP protocol for TIMM17B antibody 11062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

ROMO1 is a constituent of the human presequence translocase required for YME1L protease import.

Authors - Frank Richter
humanWB

Infect Immun

The type III system-secreted effector EspZ localizes to host mitochondria and interacts with the translocase of inner mitochondrial membrane 17b.

Authors - Shames Stephanie R SR

Sci Rep

The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly.

Authors - Lidia Wrobel
humanWB

Mol Cell

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.

Authors - Milena Vukotic
humanWB

Mol Cell

Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.

Authors - Yilin Kang
mouseWB

Cell Metab

Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells.

Authors - Zhilin Hu