|Positive WB detected in||HepG2 cells, MCF-7 cells, rat heart tissue, mouse hreat tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human ovary tumor tissue, human heart tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11123-1-AP targets Tim23 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, Megalobrama amblycephala, monkey, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Tim23 fusion protein Ag1620|
|Full Name||translocase of inner mitochondrial membrane 23 homolog (yeast)|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC062707|
|Gene ID (NCBI)||10431|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.
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