Tim23 Polyclonal antibody

Tim23 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11123-1-AP

Synonyms

PRO1197, TIM23, TIMM23



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, mouse heart tissue, MCF-7 cells, rat heart tissue, mouse skeletal muscle tissue, COLO 320 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman ovary tumor tissue, human heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11123-1-AP targets Tim23 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tim23 fusion protein Ag1620
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC062707
Gene symbol TIMM23
Gene ID (NCBI) 10431
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

Protocols

Product Specific Protocols
WB protocol for Tim23 antibody 11123-1-APDownload protocol
IHC protocol for Tim23 antibody 11123-1-APDownload protocol
IF protocol for Tim23 antibody 11123-1-APDownload protocol
IP protocol for Tim23 antibody 11123-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyIF

Nat Commun

Functional role of PGAM5 multimeric assemblies and their polymerization into filaments.

Authors - Karen Ruiz
humanWB

Arch Toxicol

Ochratoxin A causes mitochondrial dysfunction, apoptotic and autophagic cell death and also induces mitochondrial biogenesis in human gastric epithelium cells.

Authors - Qian Li
humanWB

Cell Rep

The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.

Authors - Kerong Wang
mouseWB

Chem Biol Interact

Activation of PINK1-Parkin-dependent mitophagy in Tri-ortho-cresyl phosphate-treated Neuro2a cells.

Authors - Yu Wang
mouseWB

J Cell Biochem

Parthenolide regulates oxidative stress-induced mitophagy and suppresses apoptosis through p53 signaling pathway in C2C12 myoblasts.

Authors - Yinghui Ren
mouseWB

Aging (Albany NY)

Protection of procyanidin B2 on mitochondrial dynamics in sepsis associated acute kidney injury via promoting Nrf2 nuclear translocation.

Authors - Jian-Xing Liu