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Tim23 Antibody 20 Publications

Rabbit Polyclonal| Catalog number: 11123-1-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Tim23 Antibody WB HepG2 cells 11123-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 11123-1-AP (Tim23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Tim23 Antibody WB rat heart tissue 11123-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 11123-1-AP (Tim23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Tim23 Antibody IF HepG2 cells 11123-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 11123-1-AP(Tim23 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Tim23 Antibody IHC human ovary tumor tissue 11123-1-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11123-1-AP(TIMM23 antibody) at dilution of 1:50 (under 10x lens)

Tim23 Antibody IP mouse heart tissue 11123-1-AP

IP Result of anti-Tim23 (IP:11123-1-AP, 4ug; Detection:11123-1-AP 1:1000) with mouse heart tissue lysate 3200ug.

Species specificity:
human, mouse, rat, monkey
Positive WB detected in:
HepG2 cells, Jurkat cells, mouse heart tissue, mouse skeletal muscle tissue, COLO 320 cells, MCF-7 cells, rat heart tissue
Positive IP detected in:
mouse heart tissue
Positive IHC detected in:
human ovary tumor tissue, human heart tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Tim23 fusion protein Ag1620
Full name:
translocase of inner mitochondrial membrane 23 homolog (yeast)
Calculated molecular weight:
22 kDa
Observed molecular weight:
22 kDa
GenBank accession number:
BC062707
Gene ID (NCBI):
10431
Gene symbol
TIMM23
Synonyms
PRO1197, TIM23, TIMM23

Background

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.


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