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Tim23 Polyclonal antibody

Tim23 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11123-1-AP

Synonyms

PRO1197, TIM23, TIMM23



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, mouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11123-1-AP targets Tim23 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Megalobrama amblycephala, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tim23 fusion protein Ag1620
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC062707
Gene symbol TIMM23
Gene ID (NCBI) 10431
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

Protocols

Product Specific Protocols
WB protocol for Tim23 antibody 11123-1-APDownload protocol
IF protocol for Tim23 antibody 11123-1-APDownload protocol
IP protocol for Tim23 antibody 11123-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization

Authors - Kevin Qian
mouseWB

Nature

The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.

Authors - Atsushi Hoshino
humanWB

Nature

In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.

Authors - Milica Momcilovic
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
mouseWB

Cell

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Authors - Xiaorong Zhang
humanWB

Cell Metab

An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.

Authors - Nunu Huang