TIMM44 Polyclonal antibody

TIMM44 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 13859-1-AP

Synonyms

MIMT44, TIM44, TIMM44



Tested Applications

Positive WB detected inHeLa cells, Neuro-2a cells, C6 cells, HEK-293 cells, Jurkat cells, A549 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 7 publications below

Product Information

13859-1-AP targets TIMM44 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMM44 fusion protein Ag4834
Full Name translocase of inner mitochondrial membrane 44 homolog (yeast)
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC033628
Gene symbol TIMM44
Gene ID (NCBI) 10469
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TIMM44 antibody 13859-1-APDownload protocol
IF protocol for TIMM44 antibody 13859-1-APDownload protocol
IP protocol for TIMM44 antibody 13859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Metabolism

Translocase of inner mitochondrial membrane 44 alters the mitochondrial fusion and fission dynamics and protects from type 2 diabetes.

Authors - Yu Wang
humanWB

Development

The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.

Authors - Verena Rauschenberger
humanWB

Elife

Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.

Authors - Yilin Kang
humanWB

Elife

COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.

Authors - Abhishek Aich
humanWB

Nucleic Acids Res

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Authors - Elena Lavdovskaia
humanWB

Nat Cell Biol

PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.

Authors - Shotaro Saita

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana (Verified Customer) (04-20-2019)

SDS-PAGE: 15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line 4-12% Bis-tris gradient gel Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C Blocking: SEA Block Blocking Buffer 1h Primary Ab: O/N incubation at 4C Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature. Lines on WB: 1. BioRad Precision Plus Protein standards 2 and 3. Lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: ST-Hdh mouse striatal cell line
TIMM44 Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:13859-1-AP)