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Transketolase Monoclonal antibody

Transketolase Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3E2F3

Cat no : 66016-1-Ig

Synonyms

TKT, TKT1, Transketolase

Validation Data Gallery

  • WB analysis using 66016-1-Ig

    WB analysis using 66016-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66016-1-Ig

    IF Staining of HepG2 using 66016-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66016-1-Ig(Transketolase antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HepG2 using 66016-1-Ig

    FC experiment of HepG2 using 66016-1-Ig

    1X10^6 HepG2 cells were stained with 0.2ug Transketolase antibody (66016-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHeLa cells, human liver tissue, human brain tissue, Jurkat cells, HepG2 cells
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66016-1-Ig is Transketolase Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Transketolase fusion protein Ag16779
Full Name transketolase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC009970
Gene symbol TKT
Gene ID (NCBI) 7086
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer’s disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

Protocols

Product Specific Protocols
WB protocol for Transketolase antibody 66016-1-IgDownload protocol
IHC protocol for Transketolase antibody 66016-1-IgDownload protocol
IF protocol for Transketolase antibody 66016-1-IgDownload protocol
FC protocol for Transketolase antibody 66016-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols