Transketolase Monoclonal antibody
Transketolase Monoclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3E2F3
Cat no : 66016-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, human liver tissue, human brain tissue, Jurkat cells, HepG2 cells |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 66016-1-Ig is Transketolase Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Transketolase fusion protein Ag16779 |
Full Name | transketolase |
Calculated molecular weight | 68 kDa |
Observed molecular weight | 68 kDa |
GenBank accession number | BC009970 |
Gene symbol | TKT |
Gene ID (NCBI) | 7086 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer’s disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.
Protocols
Product Specific Protocols | |
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WB protocol for Transketolase antibody 66016-1-Ig | Download protocol |
IHC protocol for Transketolase antibody 66016-1-Ig | Download protocol |
IF protocol for Transketolase antibody 66016-1-Ig | Download protocol |
FC protocol for Transketolase antibody 66016-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |