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  • KD/KO Validated

TMP21 Polyclonal antibody

TMP21 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15199-1-AP

Synonyms

P24(DELTA), p24delta, S31I125, S31III125, TMED10, Tmp 21 I, TMP21, Transmembrane protein Tmp21



Tested Applications

Positive WB detected inBxPC-3 cells, PC-3 cells, mouse pancreas tissue, Raji cells, rat pancreas tissue
Positive IHC detected inmouse brain tissue, human colon cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15199-1-AP targets TMP21 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMP21 fusion protein Ag7347
Full Name transmembrane emp24-like trafficking protein 10 (yeast)
Calculated molecular weight 25 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC001825
Gene symbol TMED10
Gene ID (NCBI) 10972
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMP21 antibody 15199-1-APDownload protocol
IHC protocol for TMP21 antibody 15199-1-APDownload protocol
IF protocol for TMP21 antibody 15199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proteome Sci

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Authors - Lancia N F Darville
humanWB,IF

BMC Cancer

The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.

Authors - Xue Yang
human,mouseWB,IF

Cell

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

pitna (Verified Customer) (12-19-2019)

Tmp21, a subunit of the p24 complex To reduce non-specific binding, blots were incubated in LiCor Buffer for an hour at room temperature. Representative images from Western blots of total homogenate and ER fractions

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)
TMP21 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:15199-1-AP)
FH

Boyan (Verified Customer) (01-25-2019)

Good. By IF, PFA fixation, it labels the protein at the Golgi.

  • Applications: Immunofluorescence,