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  • KD/KO Validated

TMEM55B Polyclonal antibody

TMEM55B Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 23992-1-AP

Synonyms

C14orf9, PtdIns 4,5 P2 4 Ptase I, TMEM55B, transmembrane protein 55B



Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, A549 cells, HepG2 cells, MCF-7 cells, rat heart tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23992-1-AP is TMEM55B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM55B fusion protein Ag21172
Full Name transmembrane protein 55B
Calculated Molecular Weight 277 aa, 29 kDa
Observed Molecular Weight 29-32 kDa
GenBank Accession NumberBC002867
Gene Symbol TMEM55B
Gene ID (NCBI) 90809
RRIDAB_2879391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM55B is also named as C14orf9 and PIP4P1. TMEM55B is originally identified as phosphatidylinositol-4,5-P24-phosphatases (PtdIns-4,5-P24-phosphatases) that catalyze dephosphorylation of PtdIns-4,5-P2 to PtdIns-5-P. The transmembrane protein TMEM55B is involved in the perinuclear clustering of lysosomes upon starvation. TMEM55B expression is upregulated upon starvation via TFEB and TFE3 activation, and loss of TMEM55B diminishes starvation-induced lysosomal clustering. TMEM55B can bind to the dynein adaptor JIP4, promoting dynein-dynactin-dependent lysosomal trafficking to the perinuclear region. TMEM55B is also suggested to contribute to lysosomal homeostasis and mTORC1 activation via the V-ATPase assembly (PMID: 33537719).

Protocols

Product Specific Protocols
WB protocol for TMEM55B antibody 23992-1-APDownload protocol
IP protocol for TMEM55B antibody 23992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nat Commun

Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation.

Authors - Michiko Shirane
  • KD Validated
mouseWB

JCI Insight

TFEB-mediated lysosomal exocytosis alleviates high fat diet-induced lipotoxicity in the kidney

Authors - Jun Nakamura
mouseWB,IP

Arterioscler Thromb Vasc Biol

Phosphatidylinositol-(4,5)-Bisphosphate Regulates Plasma Cholesterol Through LDL (Low-Density Lipoprotein) Receptor Lysosomal Degradation.

Authors - Yuanyuan Qin
  • KD Validated
humanWB

Genes Cells

TMEM55B contributes to lysosomal homeostasis and amino acid-induced mTORC1 activation.

Authors - Yutaka Hashimoto
  • KD Validated
monkeyWB

J Cell Biol

Tex2 is required for lysosomal functions at TMEM55-dependent ER membrane contact sites

Authors - Yuanjiao Du
  • KD Validated