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  • KD/KO Validated

TMEM97 Polyclonal antibody

TMEM97 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26444-1-AP

Synonyms

MAC30, Protein MAC30, TMEM97, transmembrane protein 97



Tested Applications

Positive WB detected inCaco-2 cells, mouse testis tissue
Positive IHC detected inmouse testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26444-1-AP is TMEM97 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM97 fusion protein Ag23917
Full Name transmembrane protein 97
Observed Molecular Weight 19-20 kDa
GenBank Accession NumberBC045655
Gene Symbol TMEM97
Gene ID (NCBI) 27346
RRIDAB_2880515
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TMEM97 antibody 26444-1-APDownload protocol
IHC protocol for TMEM97 antibody 26444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Chem Biol

A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.

Authors - Yu-Shiuan Cheng
human,mouse

Cell Signal

TMEM97 ablation aggravates oxidant-induced retinal degeneration.

Authors - Hongtao Shen
  • KO Validated
humanWB

J Bioenerg Biomembr

Meningioma-associated protein 30 accelerates the proliferation and invasion of hepatocellular carcinoma by modulating Wnt/GSK-3β/β-catenin signaling.

Authors - Yu Zhang
mouseWB

Life Sci Alliance

The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth

Authors - Marina Badenes
humanWB,IHC

Cell Biosci

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression

Authors - Liang Chen
mouseWB

bioRxiv

Sigma receptor ligands are potent anti-prion compounds that act independently of sigma receptor binding

Authors - Robert C C Mercer