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TMF1 Polyclonal antibody

TMF1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22212-1-AP

Synonyms

ARA160, TATA element modulatory factor, TMF, TMF1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using TMF1 Polyclonal antibody (22212-1-AP)

    WB analysis of mouse brain using 22212-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using TMF1 Polyclonal antibody (22212-1-AP)

    WB analysis of mouse colon using 22212-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TMF1 Polyclonal antibody (22212-1-AP)

    WB analysis of Jurkat using 22212-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using TMF1 Polyclonal antibody (22212-1-AP)

    WB analysis of rat brain using 22212-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMF1 Polyclonal antibody (22212-1-AP)

    IHC staining of human colon cancer using 22212-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMF1 Polyclonal antibody (22212-1-AP)

    IHC staining of human colon cancer using 22212-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using TMF1 Polyclonal antibody (22212-1-AP)

    IHC staining of human testis using 22212-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using TMF1 Polyclonal antibody (22212-1-AP)

    IHC staining of human testis using 22212-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells, mouse colon tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22212-1-AP is TMF1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMF1 fusion protein Ag17537
Full Name TATA element modulatory factor 1
Calculated Molecular Weight 1093 aa, 123 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC126123
Gene Symbol TMF1
Gene ID (NCBI) 7110
RRIDAB_2879031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMF1, also named as TMF and ARA160, is a potential coactivator of the androgen receptor. It mediates STAT3 degradation. The MW of TMF1 is about 160 kDa.

Protocols

Product Specific Protocols
WB protocol for TMF1 antibody 22212-1-APDownload protocol
IHC protocol for TMF1 antibody 22212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP,CoIP

Neurobiol Dis

The Warburg micro syndrome protein RAB3GAP1 modulates neuronal morphogenesis and interacts with axon elongation end ER-Golgi trafficking factors

Authors - Pankaj S Ghate
View Article