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  • KD/KO Validated

Thymosin beta 4 Polyclonal antibody

Thymosin beta 4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 19850-1-AP


FX, PTMB4, Seraspenide, T beta 4, TB4X, THYB4, Thymosin beta 4, thymosin beta 4, X linked, Thymosin beta-4, TMSB4, TMSB4X

Tested Applications

Positive WB detected inhuman skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19850-1-AP is Thymosin beta 4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Thymosin beta 4 fusion protein Ag13914
Full Name thymosin beta 4, X-linked
Calculated molecular weight 63 aa, 7 kDa
Observed molecular weight 5 kDa
GenBank accession numberBC001631
Gene symbol TMSB4X
Gene ID (NCBI) 7114
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thymosin beta 4 (Tβ4), encoded by TMSB4X gene, is an actin sequestering protein which plays an important role in the organization of the cytoskeleton. Numerous studies have demonstrated the effects of Tβ4 on cell migration, proliferation, apoptosis and inflammation after exogenous treatment (PMID: 22652458). Recently, novel findings provided compelling evidence that Tβ4 played a key role in facilitating tumor metastasis and angiogenesis. It has been found that Tβ4 expressed increasingly in a number of metastatic tumors, thus providing a potential target of opportunity for cancer management, especially for cancer metastasis therapy (PMID: 22856429).


Product Specific Protocols
WB protocol for Thymosin beta 4 antibody 19850-1-APDownload protocol
IHC protocol for Thymosin beta 4 antibody 19850-1-APDownload protocol
IF protocol for Thymosin beta 4 antibody 19850-1-APDownload protocol
IP protocol for Thymosin beta 4 antibody 19850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway.

Authors - Kazuyuki Tanaka

J Gene Med

Thymosin β4 suppresses CCl4 -induced murine hepatic fibrosis by down-regulating transforming growth factor β receptor-II.

Authors - Hanchao Li
  • KD Validated

Acta Biochim Biophys Sin (Shanghai)

Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation.

Authors - Dayu Huang

Sci Rep

Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma.

Authors - Li-Hsing Chi

J Mol Cell Biol

Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3.

Authors - Zhou Yizhuo Y
  • KD Validated


Single-cell proteomics reveals changes in expression during hair-cell development.

Authors - Ying Zhu