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Thymosin beta 4 Polyclonal antibody
Thymosin beta 4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 19850-1-AP
Validation Data Gallery
|Positive WB detected in||human skeletal muscle tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||mouse skeletal muscle tissue, mouse colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse skeletal muscle tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19850-1-AP is Thymosin beta 4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Thymosin beta 4 fusion protein Ag13914|
|Full Name||thymosin beta 4, X-linked|
|Calculated molecular weight||63 aa, 7 kDa|
|Observed molecular weight||5 kDa|
|GenBank accession number||BC001631|
|Gene ID (NCBI)||7114|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Thymosin beta 4 (Tβ4), encoded by TMSB4X gene, is an actin sequestering protein which plays an important role in the organization of the cytoskeleton. Numerous studies have demonstrated the effects of Tβ4 on cell migration, proliferation, apoptosis and inflammation after exogenous treatment (PMID: 22652458). Recently, novel findings provided compelling evidence that Tβ4 played a key role in facilitating tumor metastasis and angiogenesis. It has been found that Tβ4 expressed increasingly in a number of metastatic tumors, thus providing a potential target of opportunity for cancer management, especially for cancer metastasis therapy (PMID: 22856429).
|Product Specific Protocols|
|WB protocol for Thymosin beta 4 antibody 19850-1-AP||Download protocol|
|IHC protocol for Thymosin beta 4 antibody 19850-1-AP||Download protocol|
|IF protocol for Thymosin beta 4 antibody 19850-1-AP||Download protocol|
|IP protocol for Thymosin beta 4 antibody 19850-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochem Biophys Res Commun
Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway.
J Gene Med
Thymosin β4 suppresses CCl4 -induced murine hepatic fibrosis by down-regulating transforming growth factor β receptor-II.
Acta Biochim Biophys Sin (Shanghai)
Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation.
Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma.
J Mol Cell Biol
Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3.
Single-cell proteomics reveals changes in expression during hair-cell development.