TMX1 Polyclonal antibody

TMX1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 27489-1-AP

Synonyms

PDIA11, TMX, TMX1, TXNDC, TXNDC1, UNQ235/PRO268



Tested Applications

Positive WB detected inHEK-293T cells, mouse brain tissue, HeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

27489-1-AP targets TMX1 in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMX1 fusion protein Ag26595
Full Name thioredoxin-related transmembrane protein 1
Calculated molecular weight 32 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC036460
Gene symbol TMX1
Gene ID (NCBI) 81542
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMX1, one of the few transmembrane members of the family, is the first vascular thiol isomerase with antithrombotic function. It forms functional complexes with the ER lectin calnexin and preferentially intervenes during maturation of cysteine-containing, membrane-associated proteins while ignoring the same cysteine-containing ectodomains if not anchored at the ER membrane. As such, TMX1 is the first example of a topology-specific client protein redox catalyst in living cells(PMID: 30655304). Covalent modification with AMS increases the molecular mass of originally oxidized species by ~0.5 kDa per thiol group, which allows enhanced separation from the reduced form in electrophoresis(PMID: 29123984). TMX1 has 2 bands produces by redox with the MW of 30-32 kDa.

Protocols

Product Specific Protocols
WB protocol for TMX1 antibody 27489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols